Interaction between bacterial outer membrane proteins and periplasmic   quality control factors: a kinetic partitioning mechanism

doi:10.1042/BJ20110264

CORC > 北京大学 > 化学与分子工程学院

	Interaction between bacterial outer membrane proteins and periplasmic quality control factors: a kinetic partitioning mechanism
	Wu, Si ; Ge, Xi ; Lv, Zhixin ; Zhi, Zeyong ; Chang, Zengyi ; Zhao, Xin Sheng
刊名	biochemical journal
	2011
关键词	DegP fluorescence resonance energy transfer (FRET) outer membrane protein single-molecule detection Skp SurA ESCHERICHIA-COLI CHAPERONE SKP MOLECULAR CHAPERONE SUBSTRATE PROTEINS IN-VIVO SURA PROTEASE DEGP BIOGENESIS COMPLEXES
DOI	10.1042/BJ20110264
英文摘要	The OMPs (outer membrane proteins) of Gram-negative bacteria have to be translocated through the periplasmic space before reaching their final destination. The aqueous environment of the periplasmic space and high permeability of the outer membrane engender such a translocation process inevitably challenging. In Escherichia coli, although SurA, Skp and DegP have been identified to function in translocating OMPs across the periplasm, their precise roles and their relationship remain to be elucidated. In the present paper, by using fluorescence resonance energy transfer and single-molecule detection, we have studied the interaction between the OMP OmpC and these periplasmic quality control factors. The results of the present study reveal that the binding rate of OmpC to SurA or Skp is much faster than that to DegP, which may lead to sequential interaction between OMPs and different quality control factors. Such a kinetic partitioning mechanism for the chaperone-substrate interaction may be essential for the quality control of the biogenesis of OMPs; Biochemistry & Molecular Biology; SCI(E); 13; ARTICLE; 505-511; 438
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/313141]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Wu, Si,Ge, Xi,Lv, Zhixin,et al. Interaction between bacterial outer membrane proteins and periplasmic quality control factors: a kinetic partitioning mechanism[J]. biochemical journal,2011.
APA	Wu, Si,Ge, Xi,Lv, Zhixin,Zhi, Zeyong,Chang, Zengyi,&Zhao, Xin Sheng.(2011).Interaction between bacterial outer membrane proteins and periplasmic quality control factors: a kinetic partitioning mechanism.biochemical journal.
MLA	Wu, Si,et al."Interaction between bacterial outer membrane proteins and periplasmic quality control factors: a kinetic partitioning mechanism".biochemical journal (2011).