The multiple antibiotic resistance regulator MarR is a copper sensor in   Escherichia coli

doi:10.1038/NCHEMBIO.1380

CORC > 北京大学 > 化学与分子工程学院

	The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli
	Hao, Ziyang ; Lou, Hubing ; Zhu, Rongfeng ; Zhu, Jiuhe ; Zhang, Dianmu ; Zhao, Boxuan Simen ; Zeng, Shizhe ; Chen, Xing ; Chan, Jefferson ; He, Chuan ; Chen, Peng R.
刊名	自然化学生物学
	2014
关键词	DNA-BINDING MECHANISM BACTERICIDAL ANTIBIOTICS NADH DEHYDROGENASE-2 PROTEIN-STRUCTURE CRYSTAL-STRUCTURE LIVING CELLS HYDROPEROXIDE OXIDATION DEATH TOXICITY
DOI	10.1038/NCHEMBIO.1380
英文摘要	The widely conserved multiple antibiotic resistance regulator (MarR) family of transcription factors modulates bacterial detoxification in response to diverse antibiotics, toxic chemicals or both. The natural inducer for Escherichia coli MarR, the prototypical transcription repressor within this family, remains unknown. Here we show that copper signaling potentiates MarR derepression in E. coli. Copper(II) oxidizes a cysteine residue (Cys80) on MarR to generate disulfide bonds between two MarR dimers, thereby inducing tetramer formation and the dissociation of MarR from its cognate promoter DNA. We further discovered that salicylate, a putative MarR inducer, and the clinically important bactericidal antibiotics norfloxacin and ampicillin all stimulate intracellular copper elevation, most likely through oxidative impairment of copper-dependent envelope proteins, including NADH dehydrogenase-2. This membrane-associated copper oxidation and liberation process derepresses MarR, causing increased bacterial antibiotic resistance. Our study reveals that this bacterial transcription regulator senses copper(II) as a natural signal to cope with stress caused by antibiotics or the environment.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000328854900006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); PubMed; 19; ARTICLE; chuanhe@uchicago.edu; pengchen@pku.edu.cn; 1; 21-U48; 10
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/312549]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Hao, Ziyang,Lou, Hubing,Zhu, Rongfeng,et al. The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli[J]. 自然化学生物学,2014.
APA	Hao, Ziyang.,Lou, Hubing.,Zhu, Rongfeng.,Zhu, Jiuhe.,Zhang, Dianmu.,...&Chen, Peng R..(2014).The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli.自然化学生物学.
MLA	Hao, Ziyang,et al."The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli".自然化学生物学 (2014).