Study of the effect of lanthanide ions on the kinetics of glutamate   dehydrogenase by a chronoamperometric method

doi:10.1039/an9962100687

CORC > 北京大学 > 化学与分子工程学院

	Study of the effect of lanthanide ions on the kinetics of glutamate dehydrogenase by a chronoamperometric method
	Xin, WK ; Gao, XX
刊名	analyst
	1996
关键词	chronoamperometry glutamate dehydrogenase Initial rate enzyme activity lanthanide ion reduced nicotinamide adenine dinucleotide reductive amination
DOI	10.1039/an9962100687
英文摘要	A chronoamperometric method, based on the oxidation of reduced nicotinamide adenine dinucleotide (NADH) on a glassy carbon electrode, for assaying glutamate dehydrogenase activity is described, Once the reaction was initiated by addition of enzyme into a well stirred assay mixture containing NADH, the concentration of NADH and the steady-state limiting current of NADH oxidation on the electrode decreased linearly in a short time range from the start of the reaction, The rate of the enzyme-catalysed reaction can be calculated from the decrease in the current, This method is reliable for the accurate determination of enzyme activity in purified extracts from liver and crude extracts from cucumber root, The major advantage of this method is the real-time and continuous measurement of the change in coenzyme concentration, so the real initial reaction rate, v(0), can be determined, This method has been used to detect the effect of La3+, Eu3+, Yb3+ and Zn2+ ions on the enzyme activity, The results suggest that lanthanide ions can increase the activity of the enzyme in glutamate synthesis, and Zn2+ acts as a potent inhibitor of the enzyme.; Chemistry, Analytical; SCI(E); 8; ARTICLE; 5; 687-690; 121
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/258406]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xin, WK,Gao, XX. Study of the effect of lanthanide ions on the kinetics of glutamate dehydrogenase by a chronoamperometric method[J]. analyst,1996.
APA	Xin, WK,&Gao, XX.(1996).Study of the effect of lanthanide ions on the kinetics of glutamate dehydrogenase by a chronoamperometric method.analyst.
MLA	Xin, WK,et al."Study of the effect of lanthanide ions on the kinetics of glutamate dehydrogenase by a chronoamperometric method".analyst (1996).