Interactions of human serum albumin with chlorogenic acid and ferulic   acid

doi:10.1016/j.bbagen.2004.06.021

CORC > 北京大学 > 化学与分子工程学院

	Interactions of human serum albumin with chlorogenic acid and ferulic acid
	Kang, J ; Liu, Y ; Xie, MX ; Li, S ; Jiang, M ; Wang, YD
刊名	biochimica et biophysica acta general subjects
	2004
关键词	chlorogenic acid ferulic acid human serum albumin FT-IR spectroscopy fluorescence spectroscopy TRANSFORM INFRARED-SPECTROSCOPY CAFFEIC ACID FATTY-ACIDS SECONDARY STRUCTURE BINDING-SITES QUINIC ACID FLUORESCENCE PROTEIN DERIVATIVES PHENOLICS
DOI	10.1016/j.bbagen.2004.06.021
英文摘要	The interactions of chlorogenic acid and ferulic acid with human serum albumin (HSA) have been investigated by fluorescence and Fourier transformed infrared (FT-IR) spectrometry. Fluorescence results showed that one molecule of protein combined with one molecule of drugs at the molar ratio of drug to HSA ranging from 1 to 10, and their binding affinities (K-A) are 4.37 x 10(4) M-1 and 2.23 x 10(4) M-1 for chlorogenic acid and ferulic acid, respectively. The primary binding site for chlorogenic acid is most likely located on IIA and that for ferulic acid in IIIA. The main mechanism of protein fluorescence quenching was static quenching process. Combining the curve-fitting results of infrared amide I and amide III bands, the alterations of protein secondary structure after drug complexation were estimated. With increasing the drug concentration, the protein alpha-helix structure decreased gradually and the reduction of protein alpha-helix structure reached about 7% and 5% for protein binding with chlorogenic acid and ferulic acid individually at the drug to protein molar ratio of 30. This indicated a partial unfolding of HSA in the presence of the two acids. From the fluorescence and FT-IR results, the binding mode was discussed. (C) 2004 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000224166100011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); 248; ARTICLE; 2; 205-214; 1674
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/254066]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Kang, J,Liu, Y,Xie, MX,et al. Interactions of human serum albumin with chlorogenic acid and ferulic acid[J]. biochimica et biophysica acta general subjects,2004.
APA	Kang, J,Liu, Y,Xie, MX,Li, S,Jiang, M,&Wang, YD.(2004).Interactions of human serum albumin with chlorogenic acid and ferulic acid.biochimica et biophysica acta general subjects.
MLA	Kang, J,et al."Interactions of human serum albumin with chlorogenic acid and ferulic acid".biochimica et biophysica acta general subjects (2004).