HPLC analysis and pharmacokinetic study of paeoniflorin after   intravenous administration of a new frozen dry powder formulation in   rats

doi:10.1365/s10337-006-0106-5

CORC > 北京大学 > 化学与分子工程学院

	HPLC analysis and pharmacokinetic study of paeoniflorin after intravenous administration of a new frozen dry powder formulation in rats
	Cheng, Shan ; Qiu, Feng ; Wang, Shuhui ; He, Junqi
刊名	chromatographia
	2006
关键词	column liquid chromatography pharmacokinetic studies paeoniflorin in rat plasma SOLID-PHASE EXTRACTION PERFORMANCE LIQUID-CHROMATOGRAPHY PAEONIAE-RADIX EXTRACT SI-WU DECOCTION GLYCYRRHIZIN MICE ALBIFLORIN MUSCLE SERUM ROOT
DOI	10.1365/s10337-006-0106-5
英文摘要	A simple and specific high performance liquid chromatographic (HPLC) method with UV detection using picroside 11 as the internal standard was developed and validated to determine the concentration of paeoniflorin in rat plasma and study its pharmacokinetics after an single intravenous administration of 40 mg kg(-1) paeoniflorin to Wistar rats. The analytes of interest were extracted from rat plasma samples by ethyl acetate after acidification with 0.05 mol L-1 NaH2PO4 solution (pH 5.0). Chromatographic separation was achieved on an Agilent XDB C-18 column (250 x 4.6 mm I.D., 5 mu m) with a Shim-pack GVP-ODS C-18 guard column (10 x 4.6 mm I.D., 5 mu m) using a mobile phase consisting of acetonitrile-water-acetic acid (18:82:0.4, v/v/v) at a flow rate of 1.0 mL min(-1). The UV detection was performed at a wavelength of 230 nm. The linear calibration curves were obtained in the concentration range of 0.05-200.0 mu g mL(-1) in rat plasma with the lower limit of quantification (LLOQ) of 0.05 mu g mL(-1). The intra- and inter-day precisions in terms of % relative standard deviation (RSD) were lower than 5.7 and 8.2% in rat plasma, respectively. The accuracy in terms of % relative error (RE) ranged from -1.9 to 2.6% in rat plasma. The extraction recoveries of paeoniflorin and picroside 11 were calculated to be 69.7 and 56.9%, respectively. This validated method was successfully applied to the pharmacokinetic study of a new paeoniflorin frozen dry power formulation. After single intravenous administration, the main pharmacokinetic parameters t(1/2), AUC(0-infinity), CLTOT, V-Z, MRT0-infinity and V-ss were 0.739 +/- 0.232 h, 43.75 +/- 6.90 mu g h mL(-1), 15.50 +/- 2.46 L kg(-1) h(-1), 1.003 +/- 0.401 L kg(-1), 0.480 +/- 0.055 h and 0.444 +/- 0.060 L kg(-1), respectively.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); 0; ARTICLE; 11-12; 661-666; 64
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/251208]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Cheng, Shan,Qiu, Feng,Wang, Shuhui,et al. HPLC analysis and pharmacokinetic study of paeoniflorin after intravenous administration of a new frozen dry powder formulation in rats[J]. chromatographia,2006.
APA	Cheng, Shan,Qiu, Feng,Wang, Shuhui,&He, Junqi.(2006).HPLC analysis and pharmacokinetic study of paeoniflorin after intravenous administration of a new frozen dry powder formulation in rats.chromatographia.
MLA	Cheng, Shan,et al."HPLC analysis and pharmacokinetic study of paeoniflorin after intravenous administration of a new frozen dry powder formulation in rats".chromatographia (2006).