Liquid chromatography/mass spectrometry for metabonomics investigation   induced by aristolochic acid of the biochemical effects in rats: the use   of information-dependent acquisition for biomarker identification

doi:10.1002/rcm.3438

CORC > 北京大学 > 化学与分子工程学院

	Liquid chromatography/mass spectrometry for metabonomics investigation induced by aristolochic acid of the biochemical effects in rats: the use of information-dependent acquisition for biomarker identification
	Chan, Wan ; Lee, Kim-Chung ; Liu, Ning ; Wong, Ricky N. S. ; Liu, Huwei ; Cai, Zongwei
刊名	质谱学快讯
	2008
关键词	CHINESE HERBS NEPHROPATHY CONSTANT NEUTRAL LOSS MASS-SPECTROMETRY DNA-ADDUCTS INDUCED NEPHROTOXICITY REDUCTIVE ACTIVATION HPLC-TOF/MS METABOLISM URINE MS
DOI	10.1002/rcm.3438
英文摘要	The toxic effects of oral administrations of nephrotoxic and carcinogenic aristolochic acid (AA) to male Sprague-Dawley rats were investigated by using high-performance liquid chromatography coupled with a quadrupole time-of-flight mass spectrometer. Analysis of the urine and plasma samples revealed distinct changes in the biochemical patterns in the AA-dosed rats. After peak finding and alignment, principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for multivariate data analysis. Potential biomarkers were studied by high-resolution mass spectrometry (MS) and tandem mass spectrometry (MS/MS) analyses. The MS/ MS spectra of all endogenous metabolites satisfying the pre-defined criteria were acquired in a single information-dependent acquisition (IDA) analysis, demonstrating that IDA was an efficient approach for structural elucidation in metabonomic studies. Citric acid and a glucuronide-containing metabolite were observed as potential biomarkers in rat urine. A significant increase in plasma creatinine concentration was also observed in the AA-dosed rats, which indicated that AA induced an adverse effect on the renal clearance function. Copyright (C) 2008 John Wiley & Sons, Ltd.; Chemistry, Analytical; Spectroscopy; SCI(E); 0; ARTICLE; 6; 873-880; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/250618]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Chan, Wan,Lee, Kim-Chung,Liu, Ning,et al. Liquid chromatography/mass spectrometry for metabonomics investigation induced by aristolochic acid of the biochemical effects in rats: the use of information-dependent acquisition for biomarker identification[J]. 质谱学快讯,2008.
APA	Chan, Wan,Lee, Kim-Chung,Liu, Ning,Wong, Ricky N. S.,Liu, Huwei,&Cai, Zongwei.(2008).Liquid chromatography/mass spectrometry for metabonomics investigation induced by aristolochic acid of the biochemical effects in rats: the use of information-dependent acquisition for biomarker identification.质谱学快讯.
MLA	Chan, Wan,et al."Liquid chromatography/mass spectrometry for metabonomics investigation induced by aristolochic acid of the biochemical effects in rats: the use of information-dependent acquisition for biomarker identification".质谱学快讯 (2008).