急/慢性粒细胞白血病患者骨髓血细胞的FTIR研究

doi:10.3321/j.issn:0251-0790.2001.06.006

CORC > 北京大学 > 化学与分子工程学院

	急/慢性粒细胞白血病患者骨髓血细胞的FTIR研究; Fourier Transform Infrared Spectroscopic Investigation of Human Blood Cells in Bone Marrow from AML and CML Patients
	高体玉 ; 慈云祥 ; 王德炳
刊名	高等学校化学学报
	2001
关键词	FTIR 白血病骨髓血细胞 fourier transform infrared spectroscopy leukemia blood cells in bone marrow
DOI	10.3321/j.issn:0251-0790.2001.06.006
英文摘要	研究了急性粒细胞白血病(AML)和慢性粒细胞白血病(CML)患者的骨髓淋巴细胞、粒细胞和红细胞的傅立叶红外光谱(FTIR). 结果发现: (1) 这三种细胞FTIR存在着差异性. (2) AML与CML之间淋巴细胞和粒细胞的谱图差异性不明显, 但红细胞的谱图却存在可识别的差异性. 与CML红细胞相比, AML红细胞酰胺Ⅰ带较宽, AML红细胞蛋白质二级结构中α-螺旋结构的含量较低; 1 540 cm-1与1 084 cm-1吸收峰面积的比值较低, 范围较窄. 该结果提示, AML和CML骨髓血红细胞中核红细胞的数量分布不同, 同时提示骨髓红细胞的成熟可能与白血病细胞的增殖有关.; Eight samples of bone marrow coming from patients affected by acute myeloid leukemia (AML) and nine samples from patients suffered from chronic myeloid leukemia(CML) were studied by FTIR spectroscopy. Obvious differences are observed among the erythrocytes, granulocytes and lymphocytes, No significant differences are found in the granulocytes/lymphocytes between AML and CML samples whereas the spectroscopic differences of leukemic erythrocytes are observed, The ratios of the integrated areas of the band at 1 540 cm(-1) and 1 084 cm(-1) in the spectra of erythrocytes from AML are closer and lower than the corresponding ratios from CML samples. Compared to that of CML, the erythrocytes from AML show broader amide band I and have a higher percentage of alpha -helix in the secondary structures of proteins. These differences indicate the different contributions of red cells in erythrocytic series in the erythropoietic development from proerythroblast to the matured erythrocytes, The results imply the maturation of red cells in bone marrow is related to the proliferation of leukemic cells. The noticeable differences may have important implications for analyzing and evaluating leukemia.; 国家自然科学基金; 中国博士后科学基金; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 3; 6; 908-911; 22
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/247212]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	高体玉,慈云祥,王德炳. 急/慢性粒细胞白血病患者骨髓血细胞的FTIR研究, Fourier Transform Infrared Spectroscopic Investigation of Human Blood Cells in Bone Marrow from AML and CML Patients[J]. 高等学校化学学报,2001.
APA	高体玉,慈云祥,&王德炳.(2001).急/慢性粒细胞白血病患者骨髓血细胞的FTIR研究.高等学校化学学报.
MLA	高体玉,et al."急/慢性粒细胞白血病患者骨髓血细胞的FTIR研究".高等学校化学学报 (2001).