Nanocapillaries for Open Tubular Chromatographic Separations of Proteins   in Femtoliter to Picoliter Samples

doi:10.1021/ac901265t

CORC > 北京大学 > 化学与分子工程学院

	Nanocapillaries for Open Tubular Chromatographic Separations of Proteins in Femtoliter to Picoliter Samples
	Wang, Xiayan ; Cheng, Chang ; Wang, Shili ; Zhao, Meiping ; Dasgupta, Purnendu K. ; Liu, Shaorong
刊名	analytical chemistry
	2009
关键词	MICROCAPILLARY LIQUID-CHROMATOGRAPHY GREEN FLUORESCENT PROTEIN SINGLE-MOLECULE DETECTION CAPILLARY-ELECTROPHORESIS ELECTROKINETIC TRANSPORT GLASS-CAPILLARIES CATION-EXCHANGE BORE COLUMNS ID COLUMNS DNA
DOI	10.1021/ac901265t
英文摘要	We have recently examined the potential of bare nanocapillaries for free solution DNA separations and demonstrated efficiencies exceeding 10(6) theoretical plates/m. In the present work, we demonstrate the use of bare and hydroxypropylcellulose (HPC) coated open tubular nanocapillaries for protein separations. Using 1.5 mu m inner diameter (i.d.) capillary columns, hydrodynamically injecting femto- to picoliter volumes of fluorescent or fluorescent dye labeled protein samples, utilizing a pneumatically pressurized chamber containing 1.0 mM sodium tetraborate solution eluent (typically 200 psi) as the pump, and performing on-column detection using a simple laser-induced fluorescence detector, we demonstrate efficiencies of close to a million theoretical plates/m while generating single digit microliter volumes of waste for a complete chromatographic run. We achieve baseline resolution for a protein mixture consisting of transferrin, alpha-lactalbumin, insulin, and alpha-2-macroglobulin.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000269312000044&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); EI; 23; ARTICLE; 17; 7428-7435; 81
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/246936]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Xiayan,Cheng, Chang,Wang, Shili,et al. Nanocapillaries for Open Tubular Chromatographic Separations of Proteins in Femtoliter to Picoliter Samples[J]. analytical chemistry,2009.
APA	Wang, Xiayan,Cheng, Chang,Wang, Shili,Zhao, Meiping,Dasgupta, Purnendu K.,&Liu, Shaorong.(2009).Nanocapillaries for Open Tubular Chromatographic Separations of Proteins in Femtoliter to Picoliter Samples.analytical chemistry.
MLA	Wang, Xiayan,et al."Nanocapillaries for Open Tubular Chromatographic Separations of Proteins in Femtoliter to Picoliter Samples".analytical chemistry (2009).