Monolithic molecularly imprinted solid-phase extraction for the   selective determination of trace cytokinins in plant samples with liquid   chromatography-electrospray tandem mass spectrometry

doi:10.1007/s00216-012-6131-3

CORC > 北京大学 > 化学与分子工程学院

	Monolithic molecularly imprinted solid-phase extraction for the selective determination of trace cytokinins in plant samples with liquid chromatography-electrospray tandem mass spectrometry
	Du, Fuyou ; Ruan, Guihua ; Liang, Shenghua ; Xie, Fuwei ; Liu, Huwei
刊名	分析化学和生物分析化学
	2012
关键词	Cytokinins Molecularly imprinted polymer Solid-phase extraction Liquid chromatography-electrospray tandem mass spectrometry IMMUNOAFFINITY PURIFICATION QUADRUPOLE-TIME POLYMERS MICROEXTRACTION ACID BIOSYNTHESIS RECOGNITION RECEPTORS HORMONES TISSUES
DOI	10.1007/s00216-012-6131-3
英文摘要	Cytokinins (CTKs) are a class of growth-regulating hormones involved in various physiological and developmental processes. More novel analytical methods for the accurate identification and quantitative determination of trace CTKs in plants have been desired to better elucidate the roles of CTKs. In this work, a novel method based on monolithic molecularly imprinted solid-phase extraction followed by liquid chromatography-electrospray tandem mass spectrometry (mMI-SPE-LC-MS/MS) was developed for accurate determination of four CTKs in plant samples. The molecularly imprinted polymer monolith was prepared by using kinetin as the template in syringes and exhibited specific recognition ability for the four CTKs in comparison with that of non-imprinted polymer monolith. Several factors affecting the extraction performance of mMI-SPE, including the pH of loading sample solution, the nature and volume of elution solvent, the flow rate of sample loading, and sample volume, were investigated, respectively. Under the optimized conditions, the proposed mMI-SPE-LC-MS/MS method was successfully applied in the selective extraction and determination of four CTKs in plant tissues, and it offers detection limits (S/N = 3) of 104, 113, 130, and 89 pg/mL and mean recoveries of 85.9%, 79.3%, 73.5%, and 70.1% for kinetin, kinetin glucoside, trans-zeatin, and meta-topolin (mT), respectively, with the corresponding RSDs less than 15%.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); EI; 0; ARTICLE; 2; 489-501; 404
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/232341]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Du, Fuyou,Ruan, Guihua,Liang, Shenghua,et al. Monolithic molecularly imprinted solid-phase extraction for the selective determination of trace cytokinins in plant samples with liquid chromatography-electrospray tandem mass spectrometry[J]. 分析化学和生物分析化学,2012.
APA	Du, Fuyou,Ruan, Guihua,Liang, Shenghua,Xie, Fuwei,&Liu, Huwei.(2012).Monolithic molecularly imprinted solid-phase extraction for the selective determination of trace cytokinins in plant samples with liquid chromatography-electrospray tandem mass spectrometry.分析化学和生物分析化学.
MLA	Du, Fuyou,et al."Monolithic molecularly imprinted solid-phase extraction for the selective determination of trace cytokinins in plant samples with liquid chromatography-electrospray tandem mass spectrometry".分析化学和生物分析化学 (2012).