Compatible buffer for capillary electrophoresis and matrix-assisted   laser desorption/ionization mass spectrometry

doi:10.1039/c3ay40397a

CORC > 北京大学 > 化学与分子工程学院

	Compatible buffer for capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry
	Chen, Hong-Xu ; Busnel, Jean-Marc ; Qiao, Liang ; Gasilova, Natalia ; Zhang, Xin-Xiang ; Girault, Hubert H.
刊名	analytical methods
	2013
关键词	VACUUM DEPOSITION INTERFACE MALDI-MS ZONE-ELECTROPHORESIS FRACTION COLLECTION PROTEIN-ANALYSIS TIME TECHNOLOGY
DOI	10.1039/c3ay40397a
英文摘要	A compatible buffer system for coupling of capillary electrophoresis (CE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was developed. The employed interface consists of a robot to drive a silver-covered separation capillary and an AnchorChip MALDI-MS target. The outlet of the capillary is grounded and connected to the pre-deposited buffer droplet on the MALDI target to make the electric connection and allow sample crystallization for MALDI-MS. The possibility of using only one buffer already containing the matrix for MALDI-MS for separation and ionization was investigated and tested on protein and peptide samples. The results show that the proposed buffer system is suitable for CE-MALDI-MS coupling, simplifies the traditional buffer mixing steps in off-line CE-MALDI-MS protocols, and is therefore highly promising for on-line analysis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000323226800070&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; Food Science & Technology; Spectroscopy; SCI(E); EI; 3; ARTICLE; 16; 4258-4262; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/228042]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Chen, Hong-Xu,Busnel, Jean-Marc,Qiao, Liang,et al. Compatible buffer for capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry[J]. analytical methods,2013.
APA	Chen, Hong-Xu,Busnel, Jean-Marc,Qiao, Liang,Gasilova, Natalia,Zhang, Xin-Xiang,&Girault, Hubert H..(2013).Compatible buffer for capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry.analytical methods.
MLA	Chen, Hong-Xu,et al."Compatible buffer for capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry".analytical methods (2013).