protein A modified Au-graphene oxide composite as an enhanced sensing   platform for SPR-based immunoassay

doi:10.1039/c3an01553j

CORC > 北京大学 > 化学与分子工程学院

	protein A modified Au-graphene oxide composite as an enhanced sensing platform for SPR-based immunoassay
	Zhang, Jia ; Sun, Ying ; Wu, Qiong ; Zhang, Hua ; Bai, Yu ; Song, Daqian
刊名	analyst
	2013
关键词	SURFACE-PLASMON RESONANCE BIOSENSOR FUNCTIONALIZATION NANOCOMPOSITES SENSITIVITY SENSORS REDUCTION HYDRAZINE DESIGN
DOI	10.1039/c3an01553j
英文摘要	A sensitive and selective wavelength modulation surface plasmon resonance (SPR) biosensor is reported with Au nanoparticle decorated graphene oxide (GO) as an enhanced sensing platform. GO sheets possess favourable water dispersibility, good biocompatibility and high loading capacity. An Au-GO composite with the Au spheres size of 15-20 nm was synthesized and modified with staphylococcal protein A (SPA). The as-prepared composite assembles directly onto the Au film surface of the SPR sensor. Meanwhile, SPA specifically recognizes and binds the Fc portion of antibodies, contributing to highly oriented antibody immobilization on the chip surface without any antibody modification. Consequently, the biosensor based on the SPA modified Au-GO composite exhibits a satisfactory response to rabbit IgG in the concentration range of 0.1-50 mg mL(-1), while the biosensor based on the sole SPA layer for antibody immobilization shows a response in the concentration range of 1.650 mg mL(-1). Experimental results show that the SPA modified Au-GO composite can be successfully used for the signal amplification of immunosensors, thereby improving the sensitivity and obviating the need of chemical modification of the antibody.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000326393300022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); 13; ARTICLE; 23; 7175-7181; 138
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/227891]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, Jia,Sun, Ying,Wu, Qiong,et al. protein A modified Au-graphene oxide composite as an enhanced sensing platform for SPR-based immunoassay[J]. analyst,2013.
APA	Zhang, Jia,Sun, Ying,Wu, Qiong,Zhang, Hua,Bai, Yu,&Song, Daqian.(2013).protein A modified Au-graphene oxide composite as an enhanced sensing platform for SPR-based immunoassay.analyst.
MLA	Zhang, Jia,et al."protein A modified Au-graphene oxide composite as an enhanced sensing platform for SPR-based immunoassay".analyst (2013).