The immunostimulatory effect of bio-active peptide from pollen on murine   and human lymphocytes

doi:10.1016/S0047-6374(98)00063-3

CORC > 北京大学 > 化学与分子工程学院

	The immunostimulatory effect of bio-active peptide from pollen on murine and human lymphocytes
	Liu, JD ; Wang, S ; Qi, JJ ; Wang, X ; Song, YL
刊名	mechanisms of ageing and development
	1998
关键词	lymphocyte bio-active peptide immunostimulatory effect proliferative response IL-2R
DOI	10.1016/S0047-6374(98)00063-3
英文摘要	The effects of bio-active peptide (BP) from buckwheat pollen and its synthetic analogues on murine and human lymphocytes and HL-60 cells were examined. Both natural and synthetic BP had the same effect in the lymphocyte blast transformation test. The proliferative response of HL-60 cells, co-cultured for 72 h with BP, was measured by the MTT method. BP had a stimulatory effect on HL-60 cell proliferation. The optical density (OD) value was 0.339 +/- 0.015, whereas, that of the control without BP was significantly lower (0.279 +/- 0.037; P < 0.05). The CD25 (IL-2R alpha chain) positive cells of BP-stimulated human lymphocytes assessed by indirect immunofluorescence were 53.97 +/- 7.12% (1 mu g/ml) and 42.53 +/- 4.4% (10 mu g/ml), significantly higher than the control (11.77 +/- 1.88%; P < 0.001). The content of sIL-2R in the supernatant after culture with BP was determined by ELISA and was 1782.3 +/- 78.18 U/ml, lower than control 2381.2 +/- 99.9 U/ml. Taken together, our results showed that BP has an immunostimulatory effect on lymphocytes. The likely mode of BP action and the possibility of developing a novel immunomodulator are discussed. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.; Cell Biology; Geriatrics & Gerontology; SCI(E); 2; ARTICLE; 2; 125-132; 104
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/216175]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, JD,Wang, S,Qi, JJ,et al. The immunostimulatory effect of bio-active peptide from pollen on murine and human lymphocytes[J]. mechanisms of ageing and development,1998.
APA	Liu, JD,Wang, S,Qi, JJ,Wang, X,&Song, YL.(1998).The immunostimulatory effect of bio-active peptide from pollen on murine and human lymphocytes.mechanisms of ageing and development.
MLA	Liu, JD,et al."The immunostimulatory effect of bio-active peptide from pollen on murine and human lymphocytes".mechanisms of ageing and development (1998).