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	Mimicry of peroxidase by co-immobilization of 1-allylimidazole and hemin on N-isopropylacrylamide-based hydrogel
	Wang, XQ ; Li, YZ ; Chang, WB
	1999
关键词	hemin immobilization peroxidase mimicry poly-N-isopropylacrylamide IMIDAZOLE PROPYL GEL HORSERADISH-PEROXIDASE OXIDATION REACTIONS METALLOPORPHYRINS PORPHYRINS COMPLEXES POLYSTYRENE CATALYSTS OXYGEN LIGAND
英文摘要	Hemin and 1-allylimidazole (AI) were copolymerized covalently to N,N'-methylene-bis-acrylamide (MBA) cross-linked N-isopropylacrylamide (NIPAAm) hydrogel to form a hemin-based mimetic enzyme of peroxidase, i.e., poly(NIPAAm/MBA/hemin/imidazole). Its catalytic activity was studied in comparison with hemin and a similar hydrogel, poly(NIPAAm/MBA/hemin), that was prepared in the absence of AI. The evaluation was based on their activities in catalyzing oxidation of fluorogenic substrate p-hydroxyphenylacetic (p-HPA) with H2O2 as the oxidant. The results showed that the activity of poly(NIPAAm/MBA/hemin/imidazole) is 21% higher than that of poly(NIPAAm/MBA/hemin) and 102% higher than hemin. This mimetic enzyme can be used for the determination of hydrogen peroxide with a detection limit (S/N= 3) of 26 nmol l(-1). (C) 1999 Elsevier Science B.V. All rights reserved.; Chemistry, Analytical; SCI(E); CPCI-S(ISTP); 16
语种	英语
DOI标识	10.1016/S0003-2670(99)00625-X
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/214116]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, XQ,Li, YZ,Chang, WB. Mimicry of peroxidase by co-immobilization of 1-allylimidazole and hemin on N-isopropylacrylamide-based hydrogel. 1999-01-01.

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