Study on Variation of Lipids during Different Growth Phases of Living   Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass   Spectrometry

doi:10.1021/ac501596v

CORC > 北京大学 > 化学与分子工程学院

	Study on Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass Spectrometry
	Liu, Yiqun ; Zhang, Jialing ; Nie, Honggang ; Doug, Chuxia ; Li, Ze ; Zheng, Zhenggao ; Bai, Yu ; Liu, Huwei ; Zhao, Jindong
刊名	analytical chemistry
	2014
关键词	DESORPTION ELECTROSPRAY-IONIZATION DIATOM THALASSIOSIRA-PSEUDONANA SP. PCC 7002 FATTY-ACIDS SULFOQUINOVOSYL DIACYLGLYCEROL PHOTOSYSTEM-II THYLAKOID MEMBRANES BATCH CULTURE REAL-TIME PHOSPHATIDYLGLYCEROL
DOI	10.1021/ac501596v
英文摘要	Lipids are important components of cell membranes and thylakoids in cyanobacteria, and they play vital roles in various biological activities. Real-time tracing of the variation of membrane lipids can provide insights of the physiological status of cyanobacterial cells. In this work, easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was utilized to investigate the changes of acidic lipids in unicellular (Synechocystis 6803, Synechococcus 7002) and filamentous (Anabaena 7120) cyanobacteria during different growth phases. A sqdX mutant with a reduced synthesis of sulfoquinovosyl diacylglycerol (SQDG) was constructed to verify the acquired data of EASI-MS. Principal component analysis (PCA) was performed to compare the acquired data, enabling the discrimination of different species of cyanobacteria in day-to-day analysis. The results showed that the three representative cyanobacteria and their growth status can be easily determined on the basis of the lipids components detected by EASI-MS. Very interestingly, significant decreases of the ratios of SQDG/PG and dramatic changes of the unsaturation level of lipids were observed in different culture times in these cyanobacteria, and these two unique characters can be used describe the aging of cyanobacteria.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000339227400052&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); EI; 0; ARTICLE; hwliu@pku.edu.cn; jzhao@pku.edu.cn; 14; 7096-7102; 86
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/212293]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Liu, Yiqun,Zhang, Jialing,Nie, Honggang,et al. Study on Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass Spectrometry[J]. analytical chemistry,2014.
APA	Liu, Yiqun.,Zhang, Jialing.,Nie, Honggang.,Doug, Chuxia.,Li, Ze.,...&Zhao, Jindong.(2014).Study on Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass Spectrometry.analytical chemistry.
MLA	Liu, Yiqun,et al."Study on Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass Spectrometry".analytical chemistry (2014).