DNA cross-triggered cascading self-amplification artificial biochemical   circuit

doi:10.1039/c4sc03225j

CORC > 北京大学 > 化学与分子工程学院

	DNA cross-triggered cascading self-amplification artificial biochemical circuit
	Nie, Ji ; Zhao, Ming-Zhe ; Xie, Wen Jun ; Cai, Liang-Yuan ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	chemical science
	2015
关键词	HYBRIDIZATION CHAIN-REACTION STRAND-DISPLACEMENT ISOTHERMAL AMPLIFICATION ULTRASENSITIVE DETECTION DUAL-AMPLIFICATION REPLICATION ENZYME RNA MICRORNAS
DOI	10.1039/c4sc03225j
英文摘要	The construction of compact and robust artificial biochemical circuits based on nucleic acids can help researchers to understand the essential mechanisms of complex biological systems, and design sophisticated strategies for various requirements. In this study, a novel DNA cross-triggered cascading self-amplification artificial biochemical circuit was developed. Once triggered by trace amounts (as low as 2 amol) of either of two fully independent oligonucleotide factors under homogeneous isothermal conditions, the circuit simultaneously amplified both factors by 10(5)-10(7) fold, which was proved using mass spectrometry. The compact and robust circuit was successfully used to construct a multi-input Boolean logic operation and a sensitive DNA biosensor based on the dual-amplification of both the target and reporter. The circuit showed great potential for signal gain in complicated molecular programming, and flexible control of nucleic acid nanomachines in biochemical network systems and nanotechnology.; Chemistry, Multidisciplinary; SCI(E); 0; ARTICLE; zhouyl@pku.edu.cn; zxx@pku.edu.cn; 2; 1225-1229; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/206580]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Nie, Ji,Zhao, Ming-Zhe,Xie, Wen Jun,et al. DNA cross-triggered cascading self-amplification artificial biochemical circuit[J]. chemical science,2015.
APA	Nie, Ji,Zhao, Ming-Zhe,Xie, Wen Jun,Cai, Liang-Yuan,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2015).DNA cross-triggered cascading self-amplification artificial biochemical circuit.chemical science.
MLA	Nie, Ji,et al."DNA cross-triggered cascading self-amplification artificial biochemical circuit".chemical science (2015).