A concise method for the preparation of peptide and arginine-rich   peptide-conjugated antisense oligonucleotide

doi:10.1021/bc034004f

CORC > 北京大学 > 化学与分子工程学院

	A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide
	Chen, CP ; Zhang, LR ; Peng, YF ; Wang, XB ; Wang, SQ ; Zhang, LH
刊名	生物共轭化学
	2003
关键词	HUMAN TELOMERASE ACTIVITY CELLS INHIBITION MOLECULES MEMBRANE DELIVERY
DOI	10.1021/bc034004f
英文摘要	Peptide-oligonucleotide conjugates were synthesized using two strategies: a mimetic signal peptide-conjugated oligonucleotide was assembled stepwise on CPG by using 2,2-dimethyl-3-hydroxypropionic acid as a linker. To solve the precipitation problem in the coupling reaction caused by the electrostatic interaction of arginine-rich peptides and oligonucleotide, oligonucleotides were absorbed on an anion-exchange resin, and then the on-resin fragment was applied for the conjugation with arginine-rich peptide. The peptide-antisense oligonucleotides showed permeability to the cell membrane of HepG-2 cells.; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic; SCI(E); PubMed; 22; ARTICLE; 3; 532-538; 14
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/200097]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Chen, CP,Zhang, LR,Peng, YF,et al. A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide[J]. 生物共轭化学,2003.
APA	Chen, CP,Zhang, LR,Peng, YF,Wang, XB,Wang, SQ,&Zhang, LH.(2003).A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide.生物共轭化学.
MLA	Chen, CP,et al."A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide".生物共轭化学 (2003).