Achieving differentiation of single-base mutations through hairpin   oligonucleotide and electric potential control

doi:10.1016/S0956-5663(02)00249-X

CORC > 北京大学 > 化学与分子工程学院

	Achieving differentiation of single-base mutations through hairpin oligonucleotide and electric potential control
	Wei, F ; Sun, B ; Liao, W ; Ouyang, JH ; Zhao, XS
刊名	生物传感器与生物电子学
	2003
关键词	biosensor hairpin oligonucleotide electric potential gene chip impedance single-base mutation SILICON 111 SURFACES ALKYL MONOLAYERS MOLECULAR BEACONS DNA HYBRIDIZATION TRANSDUCTION RECOGNITION BIOSENSORS MISMATCHES
DOI	10.1016/S0956-5663(02)00249-X
英文摘要	A novel assay for surface DNA hybridization, which is free of sample and probe labeling, convenient and of low cost, sensitive and capable of differentiation of single-base mutations, is reported. Hairpin oligonucleotides are carefully designed as probes and are covalently attached to Si chips. Segments of the human p53 gene are chosen to demonstrate the major features of the novel technique. Impedance measurement is used to detect the hybridization. To further optimize the performance, electric potential is applied on the chip. The apparently different responses of the chip to the complementary strand and the single-base mutant are shown under electric potential control. The criteria on the design of the hairpin oligonucleotides are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.; Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology; SCI(E); EI; PubMed; 0; ARTICLE; 9; 1149-1155; 18
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/200086]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wei, F,Sun, B,Liao, W,et al. Achieving differentiation of single-base mutations through hairpin oligonucleotide and electric potential control[J]. 生物传感器与生物电子学,2003.
APA	Wei, F,Sun, B,Liao, W,Ouyang, JH,&Zhao, XS.(2003).Achieving differentiation of single-base mutations through hairpin oligonucleotide and electric potential control.生物传感器与生物电子学.
MLA	Wei, F,et al."Achieving differentiation of single-base mutations through hairpin oligonucleotide and electric potential control".生物传感器与生物电子学 (2003).