Suppression of SARS-CoV entry by peptides corresponding to heptad   regions on spike glycoprotein

doi:10.1016/j.bbrc.2004.05.046

CORC > 北京大学 > 化学与分子工程学院

	Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein
	Yuan, KH ; Yi, L ; Chen, J ; Qu, XX ; Qing, TT ; Rao, X ; Jiang, PF ; Hu, JH ; Xiong, ZK ; Nie, YC ; Shi, XL ; Wang, W ; Ling, C ; Yin, XL ; Fan, KQ ; Lai, LH ; Ding, MX ; Deng, HK
刊名	生物化学与生物物理学研究通讯
	2004
关键词	SARS-CoV entry inhibitor peptides spike protein pseudotyped virus ANGIOTENSIN-CONVERTING ENZYME-2 ACUTE RESPIRATORY SYNDROME VIRAL MEMBRANE-FUSION COILED-COIL FUNCTIONAL-CHARACTERIZATION POTENT INHIBITORS VIRUS FUSION CORONAVIRUS PROTEIN HIV-1
DOI	10.1016/j.bbrc.2004.05.046
英文摘要	Heptad repeat regions (HR1 and HR2) are highly conserved sequences located in the glycoproteins of enveloped viruses. They form a six-helix bundle structure and are important in the process of virus fusion. Peptides derived from the HR regions of some viruses have been shown to inhibit the entry of these viruses. SARS-CoV was also predicted to have HR1 and HR2 regions in the S2 protein. Based on this prediction, we designed 25 peptides and screened them using a HIV-luc/SARS pseudotyped virus assay. Two peptides, HR1-1 and HR2-18, were identified as potential inhibitors, with EC50 values of 0.14 and 1.19 muM, respectively. The inhibitory effects of these peptides were validated by the wild-type SARS-CoV assay. HR1-1 and HR2-18 can serve as functional probes for dissecting the fusion mechanism of SARS-CoV and also provide the potential of further identifying potent inhibitors for SARS-CoV entry. (C) 2004 Published by Elsevier Inc.; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 46; ARTICLE; 3; 746-752; 319
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199555]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Yuan, KH,Yi, L,Chen, J,et al. Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein[J]. 生物化学与生物物理学研究通讯,2004.
APA	Yuan, KH.,Yi, L.,Chen, J.,Qu, XX.,Qing, TT.,...&Deng, HK.(2004).Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein.生物化学与生物物理学研究通讯.
MLA	Yuan, KH,et al."Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein".生物化学与生物物理学研究通讯 (2004).