The involvement of lipid rafts in epidermal growth factor-induced   chemotaxis of breast cancer cells

doi:10.1080/10929080600990500

CORC > 北京大学 > 化学与分子工程学院

	The involvement of lipid rafts in epidermal growth factor-induced chemotaxis of breast cancer cells
	Liu, Ying ; Sun, Ronghua ; Wan, Wuzhou ; Wang, Jingna ; Oppenheim, Joost J. ; Chen, Lin ; Zhang, Ning
刊名	分子膜生物学
	2007
关键词	lipid rafts cancer metastasis chemotaxis EGFR FACTOR RECEPTOR CHOLESTEROL DEPLETION SIGNAL-TRANSDUCTION CHEMOKINE RECEPTORS MEMBRANE LOCALIZATION CAVEOLAE KINASES CYCLODEXTRIN TURNOVER
DOI	10.1080/10929080600990500
英文摘要	Metastasis is the major cause of morbidity and mortality in cancer. Recent studies reveal a role of chemotaxis in cancer cell metastasis. Epidermal growth factor receptors (EGFR) have potent chemotactic effects on human breast cancer cells. Lipid rafts, organized microdomain on plasma membranes, regulate the activation of many membrane receptors. In the current study, we investigated the role of lipid rafts in EGFR-mediated cancer cell chemotaxis. Our confocal microscopy results suggested that EGFR co-localized with GM1-positive rafts. Disrupting rafts with methyl-beta-cyclodextrin (m beta CD) inhibited EGF-induced chemotaxis of human breast cancer cells. Supplementation with cholesterol reversed the inhibitory effects. Pretreatment with mbCD also impaired directional migration of cells in an in vitro "wound healing'' assay, EGF-induced cell adhesion, actin polymerization, Akt phosphorylation and protein kinase C zeta (PKC zeta) translocation. Taken together, our study indicated that integrity of lipid rafts was critical in EGF-induced chemotaxis of human breast cancer cells.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000246833300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Cell Biology; SCI(E); PubMed; 18; ARTICLE; 2; 91-101; 24
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198410]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Ying,Sun, Ronghua,Wan, Wuzhou,et al. The involvement of lipid rafts in epidermal growth factor-induced chemotaxis of breast cancer cells[J]. 分子膜生物学,2007.
APA	Liu, Ying.,Sun, Ronghua.,Wan, Wuzhou.,Wang, Jingna.,Oppenheim, Joost J..,...&Zhang, Ning.(2007).The involvement of lipid rafts in epidermal growth factor-induced chemotaxis of breast cancer cells.分子膜生物学.
MLA	Liu, Ying,et al."The involvement of lipid rafts in epidermal growth factor-induced chemotaxis of breast cancer cells".分子膜生物学 (2007).