alpha(1A)-Adrenergic Receptor Induces Activation of Extracellular   Signal-Regulated Kinase 1/2 through Endocytic Pathway

doi:10.1371/journal.pone.0021520

CORC > 北京大学 > 化学与分子工程学院

	alpha(1A)-Adrenergic Receptor Induces Activation of Extracellular Signal-Regulated Kinase 1/2 through Endocytic Pathway
	Liu, Fei ; He, Kangmin ; Yang, Xinxing ; Xu, Ning ; Liang, Zhangyi ; Xu, Ming ; Zhao, Xinsheng ; Han, Qide ; Zhang, Youyi
刊名	plos one
	2011
关键词	PROTEIN-KINASE MYOSIN-V ALPHA(1)-ADRENOCEPTOR SUBTYPES PHOSPHATIDYLINOSITOL 3-KINASE DEPENDENT ENDOCYTOSIS MEDIATED ENDOCYTOSIS CARDIAC-HYPERTROPHY TRANSGENIC MICE BETA-ARRESTINS CELLS
DOI	10.1371/journal.pone.0021520
英文摘要	G protein-coupled receptors (GPCRs) activate mitogen-activated protein kinases through a number of distinct pathways in cells. Increasing evidence has suggested that endosomal signaling has an important role in receptor signal transduction. Here we investigated the involvement of endocytosis in alpha(1A)-adrenergic receptor (alpha(1A)-AR)-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2). Agonist-mediated endocytic traffic of alpha(1A)-AR was assessed by real-time imaging of living, stably transfected human embryonic kidney 293A cells (HEK-293A). alpha(1A)-AR was internalized dynamically in cells with agonist stimulation, and actin filaments regulated the initial trafficking of alpha(1A)-AR. alpha(1A)-AR-induced activation of ERK1/2 but not p38 MAPK was sensitive to disruption of endocytosis, as demonstrated by 4 degrees C chilling, dynamin mutation and treatment with cytochalasin D (actin depolymerizing agent). Activation of protein kinase C (PKC) and C-Raf by alpha(1A)-AR was not affected by 4 degrees C chilling or cytochalasin D treatment. U73122 (a phospholipase C [PLC] inhibitor) and Ro 31-8220 (a PKC inhibitor) inhibited alpha(1B)-AR-but not alpha(1A)-AR-induced ERK1/2 activation. These data suggest that the endocytic pathway is involved in a1A-AR-induced ERK1/2 activation, which is independent of G(q)/PLC/PKC signaling.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292142800031&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 11; ARTICLE; 6; e21520; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/193372]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Fei,He, Kangmin,Yang, Xinxing,et al. alpha(1A)-Adrenergic Receptor Induces Activation of Extracellular Signal-Regulated Kinase 1/2 through Endocytic Pathway[J]. plos one,2011.
APA	Liu, Fei.,He, Kangmin.,Yang, Xinxing.,Xu, Ning.,Liang, Zhangyi.,...&Zhang, Youyi.(2011).alpha(1A)-Adrenergic Receptor Induces Activation of Extracellular Signal-Regulated Kinase 1/2 through Endocytic Pathway.plos one.
MLA	Liu, Fei,et al."alpha(1A)-Adrenergic Receptor Induces Activation of Extracellular Signal-Regulated Kinase 1/2 through Endocytic Pathway".plos one (2011).