Weak affinity ligands selection using quartz crystal microbalance   biosensor: Multi-hydroxyl amine ligands for protein separation

doi:10.1016/j.aca.2011.07.031

CORC > 北京大学 > 化学与分子工程学院

	Weak affinity ligands selection using quartz crystal microbalance biosensor: Multi-hydroxyl amine ligands for protein separation
	Cao, Qing ; Yin, Yueyan ; Quan, Li ; Li, Na ; Li, Kean ; Liu, Feng
刊名	analytica chimica acta
	2011
关键词	Weak affinity chromatography Quartz crystal microbalance Tris(hydroxymethyl)aminomethane Serinol Ethanolamine Protein separation SURFACE-PLASMON RESONANCE HUMAN SERUM-ALBUMIN MONOLITH CHROMATOGRAPHY MASS-SPECTROMETRY BINDING LYSOZYME COLUMN TRIS(HYDROXYMETHYL)AMINOMETHANE SPECTROSCOPY RECOGNITION
DOI	10.1016/j.aca.2011.07.031
英文摘要	Multi-hydroxyl amines including tris(hydroxymethyl)aminomethane (Tris), serinol and ethanolamine were selected as weak affinity ligands using a rapid screening by quartz crystal microbalance (QCM) biosensor. Based on the specific recognition between the ligands and two proteins, lysozyme (LZM) and cytochrome c (Cyt c), a weak affinity chromatography method was developed for specific separation of the two proteins. The frontal analysis results showed that the apparent dissociation constants (K(D)) of ligand-protein complexes were all in the order of weak affinity (10(-4) M). By weak affinity columns modified with the three multi-hydroxyl amines individually, LZM and Cyt c were baseline separated as retarded peaks from non-specific protein and each other in a single cycle of loading and eluting. Moreover, the Tris-modified column typically showed the satisfactory repeatability and stability as a new type of weak affinity columns. The present strategy composed of QCM selecting and affinity chromatography separating was promising to extend the variety of weak affinity ligands and develop inexpensive specific affinity methods for separation and purification of multiple proteins on one single column. (C) 2011 Elsevier B.V. All rights reserved.; Chemistry, Analytical; SCI(E); EI; PubMed; 1; ARTICLE; 2; 250-256; 703
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/192844]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Cao, Qing,Yin, Yueyan,Quan, Li,et al. Weak affinity ligands selection using quartz crystal microbalance biosensor: Multi-hydroxyl amine ligands for protein separation[J]. analytica chimica acta,2011.
APA	Cao, Qing,Yin, Yueyan,Quan, Li,Li, Na,Li, Kean,&Liu, Feng.(2011).Weak affinity ligands selection using quartz crystal microbalance biosensor: Multi-hydroxyl amine ligands for protein separation.analytica chimica acta.
MLA	Cao, Qing,et al."Weak affinity ligands selection using quartz crystal microbalance biosensor: Multi-hydroxyl amine ligands for protein separation".analytica chimica acta (2011).