Crystal Structure Analysis of a Recombinant Predicted   Acetamidase/Formamidase from the Thermophile Thermoanaerobacter   tengcongensis

doi:10.1007/s10930-011-9387-0

CORC > 北京大学 > 化学与分子工程学院

	Crystal Structure Analysis of a Recombinant Predicted Acetamidase/Formamidase from the Thermophile Thermoanaerobacter tengcongensis
	Qian, Minxie ; Huang, Qichen ; Wu, Guangteng ; Lai, Luhua ; Tang, Youqi ; Pei, Jianfeng ; Kusunoki, Masami
刊名	蛋白质杂志
	2012
关键词	Acetamidase/formamidase Structural genomics Crystal structure PROTEIN REFINEMENT
DOI	10.1007/s10930-011-9387-0
英文摘要	The structure of acetamidase/formamidase (Amds/Fmds) from the archaeon Thermoanaerobacter tengcongensis has been determined by X-ray diffraction analysis using MAD data in a crystal of space group P2(1), with unit-cell parameters a = 41.23 (3), b = 152.88 (6), c = 100.26 (7) angstrom, beta = 99.49 (3) degrees and been refined to a crystallographic R-factor of 17.4% and R-free of 23.7%. It contains two dimers in one asymmetric unit, in which native Amds/Fmds (TE19) contains of the 32 kDa native protein. The final model consists of 4 monomer (299 amino acids residues with additional 2 expression tag amino acids residues), 5 Ca2+, 4 Zn2+ and 853 water molecules. The monomer is composed by the following: an N-domain which is featuring by three-layers beta/beta/beta; a prominent excursion between N-terminal end of strand beta(7) and beta(11), which contains four-stranded antiparallel beta sheet; an C-domain which is formed by the last 82 amino acid residues with the feature of mixed alpha/beta structure. The protein contains ion-pair Ca2+-Zn2+. The portion of three-layer beta/beta/beta along with the loops provides four protein ligands to the tightly bound Ca2+, three water molecules complete the coordination; and provides five protein ligands to the tightly bound Zn2+, one water molecule complete the coordination.; Biochemistry & Molecular Biology; SCI(E); PubMed; 0; ARTICLE; 2; 166-174; 31
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/192709]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Qian, Minxie,Huang, Qichen,Wu, Guangteng,et al. Crystal Structure Analysis of a Recombinant Predicted Acetamidase/Formamidase from the Thermophile Thermoanaerobacter tengcongensis[J]. 蛋白质杂志,2012.
APA	Qian, Minxie.,Huang, Qichen.,Wu, Guangteng.,Lai, Luhua.,Tang, Youqi.,...&Kusunoki, Masami.(2012).Crystal Structure Analysis of a Recombinant Predicted Acetamidase/Formamidase from the Thermophile Thermoanaerobacter tengcongensis.蛋白质杂志.
MLA	Qian, Minxie,et al."Crystal Structure Analysis of a Recombinant Predicted Acetamidase/Formamidase from the Thermophile Thermoanaerobacter tengcongensis".蛋白质杂志 (2012).