Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive   and stable capillary electrophoresis and capillary electrophoresis with   mass spectrometry glycan analysis

doi:10.1002/jssc.201401025

CORC > 北京大学 > 化学与分子工程学院

	Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis
	Zhang, Yi-Wei ; Zhao, Ming-Zhe ; Liu, Jing-Xin ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	journal of separation science
	2015
关键词	Capillary electrophoresis Glycan analysis Mass spectrometry Poly(vinyl alcohol) N-LINKED GLYCANS MICROCHIP ELECTROPHORESIS SAMPLE PREPARATION BASIC-PROTEINS SEPARATION COATINGS MS GLYCOPROTEINS DERIVATIVES
DOI	10.1002/jssc.201401025
英文摘要	Glycosylation plays an important role in protein conformations and functions as well as many biological activities. Capillary electrophoresis combined with various detection methods provided remarkable developments for high-sensitivity glycan profiling. The coating of the capillary is needed for highly polar molecules from complex biosamples. A poly(vinyl alcohol)-coated capillary is commonly utilized in the capillary electrophoresis separation of saccharides sample due to the high-hydrophilicity properties. A modified facile coating workflow was carried out to acquire a novel multiple-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable analysis of glycans. The migration time fluctuation was used as index in the optimization of layers and a double layer was finally chosen, considering both the effects and simplicity in fabrication. With migration time relative standard deviation less than 1% and theoretical plates kept stable during 100 consecutive separations, the method was presented to be suitable for the analysis of glycosylation with wide linear dynamic range and good reproducibility. The glycan profiling of enzymatically released N-glycans from human serum was obtained by the presented capillary electrophoresis method combined with mass spectrometry detection with acceptable results.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000350175200017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); PubMed; 6; ARTICLE; zxx@pku.edu.cn; 3; 475-482; 38
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188513]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, Yi-Wei,Zhao, Ming-Zhe,Liu, Jing-Xin,et al. Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis[J]. journal of separation science,2015.
APA	Zhang, Yi-Wei,Zhao, Ming-Zhe,Liu, Jing-Xin,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2015).Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis.journal of separation science.
MLA	Zhang, Yi-Wei,et al."Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis".journal of separation science (2015).