The NMR solution structure of AIM2 PYD domain from Mus musculus reveals a distinct α2-α3 helix conformation from its human homologues | |
Hou Xianhui ; Niu Xiaogang | |
刊名 | 生物化学与生物物理学研究通讯 |
2015 | |
关键词 | AIM2,Inflammasome,NMR,PYD,Self-association,Solution structure |
DOI | 10.1016/j.bbrc.2015.04.046 |
英文摘要 | The inflammasome is a key component of the innate immune system providing the initial defense against invading organisms. Failure of inflammasome formation is the main reason for many innate and acquired immune diseases. Cytosolic protein absent in melanoma 2 (AIM2) has been reported to play an essential role in double-stranded DNA (dsDNA) sensing and inflammasome formation in response to viruses or bacteria infection. The N-terminal pyrin domain (PYD) of AIM2 interacts with the ASC PYD domain, and then recruits downstream proteins to assemble the AIM2 inflammasome. The molecular mechanisms of PYD mediated signaling remain elusive as limited structural information on PYD family. Herein, we characterized the solution structure of mouse AIM2 PYD domain by NMR spectroscopy, and compared it with the crystal structures of its two human homologues. The comparison shows mAIM2 PYD adopts a unique α2-α3 helix conformation distinct from its human homologues, but similar to the pyrin domain of human NLRP10/PYNOD, which belongs to another family. In addition, the aggregation of mAIM2 PYD domain, with the increased salt concentration, reveals that both the charge surface and hydrophobic interaction play important roles in the self-association of mAIM2 PYD.Copyright ? 2015 Elsevier Inc. All rights reserved.; SCI(E); PubMed; 0; ARTICLE; niuxg@pku.edu.cn; 2; 396-400; 461 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/188063] |
专题 | 化学与分子工程学院 |
推荐引用方式 GB/T 7714 | Hou Xianhui,Niu Xiaogang. The NMR solution structure of AIM2 PYD domain from Mus musculus reveals a distinct α2-α3 helix conformation from its human homologues[J]. 生物化学与生物物理学研究通讯,2015. |
APA | Hou Xianhui,&Niu Xiaogang.(2015).The NMR solution structure of AIM2 PYD domain from Mus musculus reveals a distinct α2-α3 helix conformation from its human homologues.生物化学与生物物理学研究通讯. |
MLA | Hou Xianhui,et al."The NMR solution structure of AIM2 PYD domain from Mus musculus reveals a distinct α2-α3 helix conformation from its human homologues".生物化学与生物物理学研究通讯 (2015). |
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