Solution Structure of Human zeta-COP: Direct Evidences for Structural   Similarity between COP I and Clathrin-Adaptor Coats

doi:10.1016/j.jmb.2008.12.083

CORC > 北京大学 > 化学与分子工程学院

	Solution Structure of Human zeta-COP: Direct Evidences for Structural Similarity between COP I and Clathrin-Adaptor Coats
	Yu, Wenyu ; Lin, Jian ; Jin, Changwen ; Xia, Bin
刊名	分子生物学杂志
	2009
关键词	COP I F-subcomplex zeta-COP AP NMR APPENDAGE DOMAIN DEPENDENT ENDOCYTOSIS ENDOPLASMIC-RETICULUM VESICLE TRANSPORT PROTEIN COMPLEXES CRYSTAL-STRUCTURE NOE ASSIGNMENT BETA-COP COATOMER NMR
DOI	10.1016/j.jmb.2008.12.083
英文摘要	COP-T-coated vesicles are protein and lipid carriers that mediate intra-Golgi transport and transport from the cis-Golgi complex to the endoplasmic reticulum in cells. The coatomer of the vesicles coat is comprised of seven subunits: alpha-COP, epsilon-COP, beta'-COP, beta-COP, gamma-COP, delta-COP, and zeta-COP. Here we report the solution structure of a truncated form (residues 1-149; zeta-COP149) of human zeta-COP (total 177 residues). It is the first three-dimensional structure. of a "core" subunit of the COP I F-subcomplex. The structure of zeta-COP149 mainly consists of a disordered N-terminal tail, a five-stranded antiparallel beta-sheet, a two-stranded antiparallel beta-sheet, and five a-helices. The global folding of zeta-COP149 is very similar to the crystal structures of API-sigma 1 and AP2-sigma 2, directly demonstrating the structural similarity between the "core" subunits of the COP I F-subcomplex and adaptor protein complexes. Through structural comparison and mutagenesis study, we have also demonstrated that the heterodimers of zeta-COP149 and gamma-COP have packing interfaces and relative subunit orientations similar to those of AP2-sigma 2 and AP2-alpha heterodimers. These results provide direct evidence supporting the previous proposal that the COP I F-subcomplex and adaptor protein complexes have similar tertiary and quaternary structures. (C) 2009 Elsevier Ltd. All rights reserved.; Biochemistry & Molecular Biology; SCI(E); PubMed; 15; ARTICLE; 4; 903-912; 386
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162581]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Yu, Wenyu,Lin, Jian,Jin, Changwen,et al. Solution Structure of Human zeta-COP: Direct Evidences for Structural Similarity between COP I and Clathrin-Adaptor Coats[J]. 分子生物学杂志,2009.
APA	Yu, Wenyu,Lin, Jian,Jin, Changwen,&Xia, Bin.(2009).Solution Structure of Human zeta-COP: Direct Evidences for Structural Similarity between COP I and Clathrin-Adaptor Coats.分子生物学杂志.
MLA	Yu, Wenyu,et al."Solution Structure of Human zeta-COP: Direct Evidences for Structural Similarity between COP I and Clathrin-Adaptor Coats".分子生物学杂志 (2009).