Sequence analysis of peptides with biological activities using   electrospray-Fourier transform ion cyclotron resonance mass spectrometry

CORC > 北京大学 > 化学与分子工程学院

	Sequence analysis of peptides with biological activities using electrospray-Fourier transform ion cyclotron resonance mass spectrometry
	He, MY ; Xu, JX ; He, XR ; Chan, TWD ; Lau, RLC
刊名	科学通报英文版
	2001
关键词	peptide with biological activity Fourier transform ion cyclotron resonance mass spectrometry electrospray collision-induced dissociation LASER-DESORPTION IONIZATION GLYCOSYLATION SITES LARGE MOLECULES RESOLUTION DISSOCIATION PROTEINS
英文摘要	(T) he mass spectra of five peptides with biological activities are reported. All mass spectra were recorded using a 4.7-T Fourier transform ion cyclotron resonance mass spectrometer equipped with an external electrospray source. The accurate molecular weights for the five peptides prepared by solid phase synthesis were measured as 1765.9013, 1063.5420, 1092.5254, 820.3804 and 1078.5193, respectively. All the data were obtained with the external calibration. Differences between observed and theoretical monoisotopic molecular weights were in the (0.2-1.0)x10(-6) range. The complete primary sequence for the five polypeptides were determined using the method of in-source electrospray ionization/collision induced dissociation (ESI/CID). All the intact y series ions and b series ions were obtained from various peptides respectively, thus determining the sequences of the five polypeptides. We found that the measured accurate molecular mass of sample 4 was not in agreement with that expected from the planned synthetic peptide. The sequences of sample 4 were determined through analysis. The corresponding accurate masses of b series ions and y series ions were gained, which proved that it was correct to re-determine the sequences.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000171106200010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 0; ARTICLE; 15; 1281-1285; 46
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/151686]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	He, MY,Xu, JX,He, XR,et al. Sequence analysis of peptides with biological activities using electrospray-Fourier transform ion cyclotron resonance mass spectrometry[J]. 科学通报英文版,2001.
APA	He, MY,Xu, JX,He, XR,Chan, TWD,&Lau, RLC.(2001).Sequence analysis of peptides with biological activities using electrospray-Fourier transform ion cyclotron resonance mass spectrometry.科学通报英文版.
MLA	He, MY,et al."Sequence analysis of peptides with biological activities using electrospray-Fourier transform ion cyclotron resonance mass spectrometry".科学通报英文版 (2001).