Combination of dynamic pH junction with capillary electrophoresis-mass   spectrometry for the determination of systemins in plant samples

doi:10.1002/elps.201300434

CORC > 北京大学 > 化学与分子工程学院

	Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples
	Bai, Yu ; Chang, Cuilan ; Du, Fuyou ; Tan, Zhijing ; Bai, Yu ; Liu, Huwei
刊名	electrophoresis
	2014
关键词	Capillary electrophoresis Dynamic pH junction Phytohormone Quadrupole time-of-flight mass spectrometry Systemin DEFENSE GENE-EXPRESSION ONLINE PRECONCENTRATION ZONE ELECTROPHORESIS WOUND SIGNAL PURIFICATION NUCLEOTIDES STACKING TIME
DOI	10.1002/elps.201300434
英文摘要	Systemin is an important group of plant peptide hormones participating in the regulation of plant defensive responses. An improved method, based on dynamic pH junction and capillary electrophoresis-quadrupole time-of-flight mass spectrometry, was developed for online enrichment and sensitive determination of trace systemins in plants. After optimization, the online enrichment factors for six target systemins ranged from 90- to 127-fold. The detection limits reached lower than 0.5 nM, which were comparable with the sensitivity of LC-MS method. Satisfactory quantitative results were obtained in terms of linearity (R-2 >= 0.993), dynamic range (3-120 ng/mL), and reproducibility (<= 6.7%). For the analysis of real plant samples, a rapid sample preparation method was developed, using two steps of SPE purification with different retention and separation mechanisms. Finally, this method realized the successful detection of tomato systemin and tobacco hydroxyproline-rich systemin I from plant leaves with shorter analysis time.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000339661300007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Chemistry, Analytical; SCI(E); 3; ARTICLE; hwliu@pku.edu.cn; 14; 1984-1988; 35
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/151403]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Bai, Yu,Chang, Cuilan,Du, Fuyou,et al. Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples[J]. electrophoresis,2014.
APA	Bai, Yu,Chang, Cuilan,Du, Fuyou,Tan, Zhijing,Bai, Yu,&Liu, Huwei.(2014).Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples.electrophoresis.
MLA	Bai, Yu,et al."Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples".electrophoresis (2014).