Conformation-Dependent Formation of the G[8-5]U Intrastrand Cross-Link   in 5-Bromouracil-Containing G-Quadruplex DNA Induced by UVA Irradiation

doi:10.1021/bi901861w

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	Conformation-Dependent Formation of the G[8-5]U Intrastrand Cross-Link in 5-Bromouracil-Containing G-Quadruplex DNA Induced by UVA Irradiation
	Lin, Guangxin ; Zhang, Jing ; Zeng, Yu ; Luo, Hai ; Wang, Yinsheng
刊名	biochemistry us
	2010
关键词	HUMAN TELOMERIC SEQUENCE IN-VITRO REPLICATION GUANINE-THYMINE UVRABC NUCLEASE POLYMERASE-ETA HUMAN GENOME K+ SOLUTION G-TETRAPLEX G-QUARTET LESION
DOI	10.1021/bi901861w
英文摘要	G-quadruplex motifs are are known to be present in telomeres of human and other organisms. Recent bioinformatic Studies also revealed the widespread existence of these motifs in promoter regions of human genes. Treatment of cultured cells with 5-bromo-2'-deoxyuridine ((Br)dU) is known to result In the substitution of DNA thymidine with (Br)dU; such replacement has been shown to sensitize cells to killing induced by UV light Our previous Studies revealed that the exposure of (Br)dU-carrying duplex DNA or (Br)dU-treated MCF-7 cells to UVB light could lead to the facile formation of intrastrand cross-link products Initiated front (Br)dU. Here we found that the exposure of (Br)dU-bearing, G-quadruplex DNA to UVA light Could also give rise to the efficient formation of the G[8-5]U intrastrand cross-link, Where the C8 of guanine in the external G-tetrad Is covalently linked with the C5 of its adjacent 3' uracil in the loop region. In addition, the yield for the cross-link product Is dependent oil the conformation of the G-quadruplex. Together, the formation of intrastrand cross-link in G-quadruplex motifs may account, for the photocytotoxic effect induced by (Br)dU incorporation, and the (Br)dU-mediated photo-cross-linking may constitute a useful method for monitoring the different conformations of G-quadruplex folding.; Biochemistry & Molecular Biology; SCI(E); EI; 8; ARTICLE; 11; 2346-2350; 49
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/151180]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Lin, Guangxin,Zhang, Jing,Zeng, Yu,et al. Conformation-Dependent Formation of the G[8-5]U Intrastrand Cross-Link in 5-Bromouracil-Containing G-Quadruplex DNA Induced by UVA Irradiation[J]. biochemistry us,2010.
APA	Lin, Guangxin,Zhang, Jing,Zeng, Yu,Luo, Hai,&Wang, Yinsheng.(2010).Conformation-Dependent Formation of the G[8-5]U Intrastrand Cross-Link in 5-Bromouracil-Containing G-Quadruplex DNA Induced by UVA Irradiation.biochemistry us.
MLA	Lin, Guangxin,et al."Conformation-Dependent Formation of the G[8-5]U Intrastrand Cross-Link in 5-Bromouracil-Containing G-Quadruplex DNA Induced by UVA Irradiation".biochemistry us (2010).