Electrospray ionization mass spectrometry probing of binding affinity of   berbamine, a flexible cyclic alkaloid from traditional Chinese medicine,   with G-quadruplex DNA

doi:10.1002/rcm.6763

CORC > 北京大学 > 化学与分子工程学院

	Electrospray ionization mass spectrometry probing of binding affinity of berbamine, a flexible cyclic alkaloid from traditional Chinese medicine, with G-quadruplex DNA
	Tan, Wei ; Zhou, Jiang ; Yuan, Gu
刊名	质谱学快讯
	2014
关键词	ONCOGENIC PROMOTER CIRCULAR-DICHROISM HUMAN GENOME RECOGNITION SEQUENCE LIGANDS REGION STABILIZATION SCAFFOLD GENE
DOI	10.1002/rcm.6763
英文摘要	RATIONALEClassic G-quadruplex binders typically have a large aromatic core and interact with G-quadruplexes through - stacking with the quartets. There are rather few reports on natural flexible cyclic molecule from traditional Chinese medicine which has high binding affinity with G-quadruplex. METHODSElectrospray ionization mass spectrometry (ESI-MS) experiments were performed to evaluate the binding affinities of a natural alkaloid, berbamine, with seven G-quadruplexes. Furthermore, we utilized autodock4 analysis to uncover the binding mode of berbamine with the G-quadruplex. RESULTSESI-MS experiments showed that berbamine has the best binding affinity toward the (GGA)(8)G-quadruplex compared with the other six G-quadruplexes. Autodock4 analysis indicated that berbamine interacted with the (GGA)(8)G-quadruplex through hydrogen bonding and van der Waals forces with a binding site at the lateral groove formed by DG8-DA9-DA15-DG16. CONCLUSIONSIn this study, we discovered a novel G-quadruplex binder, berbamine, which has high binding affinity toward the (GGA)(8)G-quadruplex. This study provided important clues regarding the probing of small molecule from traditional Chinese medicine which can target the G-quadruplex with high affinity. Copyright (c) 2013 John Wiley & Sons, Ltd.; Biochemical Research Methods; Chemistry, Analytical; Spectroscopy; SCI(E); 2; ARTICLE; zhoujiang@pku.edu.cn; guyuan@pku.edu.cn; 1; 143-147; 28
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150603]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Tan, Wei,Zhou, Jiang,Yuan, Gu. Electrospray ionization mass spectrometry probing of binding affinity of berbamine, a flexible cyclic alkaloid from traditional Chinese medicine, with G-quadruplex DNA[J]. 质谱学快讯,2014.
APA	Tan, Wei,Zhou, Jiang,&Yuan, Gu.(2014).Electrospray ionization mass spectrometry probing of binding affinity of berbamine, a flexible cyclic alkaloid from traditional Chinese medicine, with G-quadruplex DNA.质谱学快讯.
MLA	Tan, Wei,et al."Electrospray ionization mass spectrometry probing of binding affinity of berbamine, a flexible cyclic alkaloid from traditional Chinese medicine, with G-quadruplex DNA".质谱学快讯 (2014).