Solution structure and conformational heterogeneity of acylphosphatase   from Bacillus subtilis

doi:10.1016/j.febslet.2010.04.069

CORC > 北京大学 > 化学与分子工程学院

	Solution structure and conformational heterogeneity of acylphosphatase from Bacillus subtilis
	Hu, Jicheng ; Li, Dan ; Su, Xiao-Dong ; Jin, Changwen ; Xia, Bin
刊名	欧洲生化学会联合会快报
	2010
关键词	Acylphosphatase Solution structure Multiple conformation Conformational selection Nuclear magnetic resonance PROTEIN-TYROSINE-PHOSPHATASE HORSE-MUSCLE ACYLPHOSPHATASE CRYSTAL-STRUCTURE AMYLOID FORMATION 3-DIMENSIONAL STRUCTURE PYROCOCCUS-HORIKOSHII CATALYTIC MECHANISM BINDING PUMP AGGREGATION
DOI	10.1016/j.febslet.2010.04.069
英文摘要	Acylphosphatase is a small enzyme that catalyzes the hydrolysis of acyl phosphates. Here, we present the solution structure of acylphosphatase from Bacillus subtilis (BsAcP), the first from a Gram-positive bacterium. We found that its active site is disordered, whereas it converted to an ordered state upon ligand binding. The structure of BsAcP is sensitive to pH and it has multiple conformations in equilibrium at acidic pH (pH < 5.8). Only one main conformation could bind ligand, and the relative population of these states is modulated by ligand concentration. This study provides direct evidence for the role of ligand in conformational selection. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; Cell Biology; SCI(E); PubMed; 3; ARTICLE; 13; 2852-2856; 584
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150074]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Hu, Jicheng,Li, Dan,Su, Xiao-Dong,et al. Solution structure and conformational heterogeneity of acylphosphatase from Bacillus subtilis[J]. 欧洲生化学会联合会快报,2010.
APA	Hu, Jicheng,Li, Dan,Su, Xiao-Dong,Jin, Changwen,&Xia, Bin.(2010).Solution structure and conformational heterogeneity of acylphosphatase from Bacillus subtilis.欧洲生化学会联合会快报.
MLA	Hu, Jicheng,et al."Solution structure and conformational heterogeneity of acylphosphatase from Bacillus subtilis".欧洲生化学会联合会快报 (2010).