N-acetylcysteine induced quenching of red fluorescent   oligonucleotide-stabilized silver nanoclusters and the application in   pharmaceutical detection

doi:10.1016/j.aca.2013.07.037

CORC > 北京大学 > 化学与分子工程学院

	N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection
	Wang, Xinyi ; Lin, Ruoyun ; Xu, Zhihan ; Huang, Hongduan ; Li, Limei ; Liu, Feng ; Li, Na ; Yang, Xiaoda
刊名	analytica chimica acta
	2013
关键词	Fluorescence Quenching Silver nanoclusters Nucleotide N-acetylcysteine ACETYL-L-CYSTEINE LABEL-FREE AG NANOCLUSTERS DNA ACID FLUOROPHORES DISULFIDES CLUSTERS PROTEIN THIOLS
DOI	10.1016/j.aca.2013.07.037
英文摘要	In this work, we reported a new, simple and sensitive method for determination of N-acetylcysteine (NAC) based on quenching of the red fluorescence of oligonuleotide-protected silver nanoculsters (Ag NCs) with the quantum yield of 68.3 +/- 0.3%. This method was successfully used for the assay of NAC granules presenting a linear range from 100 nM to 1200 nM (LOD of 50 nM) with minimal interferences from potential coexisting substances. It is for the first time that quenching performance of the thiol-containing compound was found to follow a non-linear Stern-Volmer profile, indicative of a complicated quenching mechanism with static quenching dominating, in which DNA-template of Ag NCs was partly replaced by NAC, as elucidated by spectral investigations. This study extended the analytical application of silver nanoclusters as well as provided a more insightful understanding of the quenching mechanism of thiol-compounds on the fluorescence of Ag NCs. (c) 2013 Elsevier B.V. All rights reserved.; Chemistry, Analytical; SCI(E); EI; PubMed; 4; ARTICLE; 79-85; 793
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149779]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Xinyi,Lin, Ruoyun,Xu, Zhihan,et al. N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection[J]. analytica chimica acta,2013.
APA	Wang, Xinyi.,Lin, Ruoyun.,Xu, Zhihan.,Huang, Hongduan.,Li, Limei.,...&Yang, Xiaoda.(2013).N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection.analytica chimica acta.
MLA	Wang, Xinyi,et al."N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection".analytica chimica acta (2013).