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	利用噬菌体展示技术筛选放射性标记肺癌靶肽YC11及其生物分布; Screening, Radiolabeling and Biodistribution of Lung Tumor-targeting Peptide YC11 by in vivo Phage Display
	梁坤 ; 褚泰伟
	2013
关键词	噬菌体展示 靶肽 肺癌 生物分布 放射性标记 Phage display Targeting peptide Lung tumor Biodistribution Radiolabeling
英文摘要	利用体内噬菌体展示技术筛选了肿瘤特异性结合靶肽,获得了肺腺癌靶肽CSIHYPLSC(YC11)并对其进行了放射性碘标记.采用反相高效液相色谱技术(RP-HPLC)分离纯化131I-YC11和对照肽131I-NGR,放化纯度大于99%.在荷人肺腺癌A549裸鼠体内的分布结果表明,131I-YC11的肿瘤/血液放射性计数比为0.62,肿瘤/心脏放射性计数比为2.06,肿瘤/肺放射性计数比为1.11,肿瘤/肝脏放射性计数比为0.81,略高于131I-NGR的对应值.131I-YC11在肿瘤中的摄取值为2.79%ID/g,与131I-NGR在肿瘤中的摄取值(1.61%ID/g)相比,131I-YC11...; In vivo phage display was used to isolate tumor-targeting peptides. A cycloheptapeptide CSIHY-PLSC(YC11) ligand targeting A549 human lung tumor was identified and radioiodinated. The labeled complex I-131-YC11 and I-131-NGR(control peptide) were isolated and purified by reverse phase high-performance liquid chromatography(RP-HPLC). The radiochemical purity was estimated to be better than 99%. The bio-distribution results of I-131-YC11 and control peptide I-131-NGR in mice bearing A549 tumor demonstrated that the tumor-to-blood, tumor-to-heart, tumor-to-lung and tumor-to-liver uptake ratios of I-131-YC11 were 0. 62, 2. 06, 1. 11 and 0. 81, respectively, and slightly higher than those of I-131-NGR. The uptake of I-131-YC11 in tumor was 2. 79% ID/g, more than that of I-131-NCR (1.61% ID/g). I-131-YC11 was more concentrated in A549 tumor than I-131-NGR. Conclusively, YC11 could be a potential lung cancer targeting agent.; SCI(E); EI; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; 04; 870-874; 34
语种	中文
出处	EI ; SCI ; 知网 ; 万方
出版者	高等学校化学学报
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/79454]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	梁坤,褚泰伟. 利用噬菌体展示技术筛选放射性标记肺癌靶肽YC11及其生物分布, Screening, Radiolabeling and Biodistribution of Lung Tumor-targeting Peptide YC11 by in vivo Phage Display. 2013-01-01.

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