| 花粉多肽对钙调素的拮抗作用研究; Studies of pollen peptide antagonising effect on calmodulin | |
| 曲泽川 ; 宋艳玲 ; 阎虎生 | |
| 1999 | |
| 关键词 | 花粉多肽 钙调素 钙调素拮抗剂 pollen peptide calmodulin calmodulin antagonist |
| 英文摘要 | 研究了在Ｃａ２＋ 存在下荞麦花粉肽及其类似物和丹磺酰标记的钙调素（Ｄ－ＣａＭ）的相互作用， 结果表明， 除肽ＢＰ－１ 外， 都能与Ｄ－ＣａＭ 结合而形成复合物． 利用荧光光谱法测定了这些复合物的解离常数Ｋｄ． 在所研究的多肽中以ＢＰ－１３ 拮抗ＣａＭ 作用最强， 其Ｋｄ 值为４．６×１０－ ２μｍ ｏｌ／Ｌ， 抑制钙依赖性磷酸二酯酶活性的ＩＣ５０为２．２ μｍ ｏｌ／Ｌ． 我们还发现， 当Ｄ－丙氨酸残基取代没有亲和性的ＢＰ－１ 中甘氨酸残基时， 其亲和性明显提高．; The interactions of the buckwheat pollen peptide and its analogues with dansyl-labeled calmodulin(D-CaM) were studied in the presence of Ca2+. All peptides except BP-I can associate with D-CaM to form complexes peptide-calmodulin. The dissociation constants Kd for the complexes were determined using the fluorescence spectra. Among the tested peptides, BP-13 showed a stronger inhibitory effect on CaM, with IC50 of 2, 2 mu mol/L and K-d Of 4. 6 X 10(-2) mu mol/L. The effects of the hydrophobicity and the behavior being predicated to have a higher alpha-helical propensities on their affinities were further studied. We have found that D-alanine substitution in BP-1 results in affinity enhancement, The result will be helpful to improving our ability to design peptides possessing anticalmodulin activity.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000083723600018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); EI; 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 2; 11; 1738-1742; 20 |
| 语种 | 中文 |
| 出处 | 知网 ; 万方 ; SCI |
| 出版者 | 高等学校化学学报 |
| 内容类型 | 其他 |
| 源URL | [http://hdl.handle.net/20.500.11897/78528]  |
| 专题 | 化学与分子工程学院 |
| 推荐引用方式 GB/T 7714 | 曲泽川,宋艳玲,阎虎生. 花粉多肽对钙调素的拮抗作用研究, Studies of pollen peptide antagonising effect on calmodulin. 1999-01-01. |
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