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	Segmentation and classification of two-channel C. elegans nucleus-labeled fluorescence images
	Zhao, Mengdi ; An, Jie ; Li, Haiwen ; Zhang, Jiazhi ; Li, Shang-Tong ; Li, Xue-Mei ; Dong, Meng-Qiu ; Mao, Heng ; Tao, Louis
	2017
关键词	C. elegans Nucleus Aging Two-channel fluorescence image Morphology Segmentation Classification AGE-RELATED-CHANGES AFFECT LIFE-SPAN CAENORHABDITIS-ELEGANS CELL SEGMENTATION ARCHITECTURE MORPHOLOGY INTEGRITY
英文摘要	Background: Aging is characterized by a gradual breakdown of cellular structures. Nuclear abnormality is a hallmark of progeria in human. Analysis of age-dependent nuclear morphological changes in Caenorhabditis elegans is of great value to aging research, and this calls for an automatic image processing method that is suitable for both normal and abnormal structures. Results: Our image processing method consists of nuclear segmentation, feature extraction and classification. First, taking up the challenges of defining individual nuclei with fuzzy boundaries or in a clump, we developed an accurate nuclear segmentation method using fused two-channel images with seed-based cluster splitting and k-means algorithm, and achieved a high precision against the manual segmentation results. Next, we extracted three groups of nuclear features, among which five features were selected by minimum Redundancy Maximum Relevance (mRMR) for classifiers. After comparing the classification performances of several popular techniques, we identified that Random Forest, which achieved a mean class accuracy (MCA) of 98.69%, was the best classifier for our data set. Lastly, we demonstrated the method with two quantitative analyses of C. elegans nuclei, which led to the discovery of two possible longevity indicators. Conclusions: We produced an automatic image processing method for two-channel C. elegans nucleus-labeled fluorescence images. It frees biologists from segmenting and classifying the nuclei manually.; Ministry of Science and Technology of China [2011CB809105, 2014CB84980001]; Natural Science Foundation of China [91232715, 61101156, 61421062, 61520106004, 61375022]; State Key Laboratory of Cognitive Neuroscience and Learning [CNLZD1404]; Beijing Municipal Science and Technology Commission [Z151100000915070]; SCI(E); ARTICLE; 18
语种	英语
出处	SCI
出版者	BMC BIOINFORMATICS
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/470852]
专题	数学科学学院 生命科学学院
推荐引用方式 GB/T 7714	Zhao, Mengdi,An, Jie,Li, Haiwen,et al. Segmentation and classification of two-channel C. elegans nucleus-labeled fluorescence images. 2017-01-01.

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