Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study

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	Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study
	Cao, Hongjuan; Feng, Lifang; Wu, Zhenxian; Hou, Wentao; Li, Shujun; Hao, Yulin; Wu, Lin; Wu, L (reprint author), China Med Univ, Sch Stomatol, Dept Prosthodont, Shenyang, Liaoning, Peoples R China.
刊名	ELSEVIER SCIENCE BV
	2017-11-01
卷号	80 页码:7-17
关键词	Low Intensity Pulsed Ultrasound (Lipus) Porous Ti6ai4v Scaffolds Osteoblasts Biological Behavior Bone Formation
ISSN号	0928-4931
英文摘要	Low-intensity pulsed ultrasound (LIPUS) has been used in patients with fresh fractures, delayed union and nonunion to enhance bone healing and improve functional outcome. However, there were few studies concerning the effects of LIPUS on the biological behavior of osteoblasts on porous scaffolds. This study aimed to evaluate the effects of LIPUS on the biological behavior of osteoblasts on porous titanium-6aluminum-4vanadium (Ti6A14V) alloy scaffolds in vitro and in vivo. Scaffolds were randomly divided into an ultrasound group and a control group. Mouse pre-osteoblast cells were cultured with porous Ti6AI4V scaffolds in vitro. The effects of LIPUS on the biological behavior of osteoblasts were evaluated by observing the adhesion, proliferation, differentiation and ingrowth depth on porous Ti6A14V scaffolds. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated via micro-CT, fluorescent staining and toluidine blue staining. The results revealed that osteoblast adhered well to the scaffolds, and there was no significant difference in the methyl thiazolyl tetrazolium value between the ultrasound group and the control group (p > 0.05). Compared with the control group, ultrasound promoted the alkaline phosphatase activity, osteocalcin levels and ingrowth depth of the cells on the scaffolds (p < 0.05). In addition, micro-CT and histomorphological analysis showed that the volume and amount of new bone formation were increased and that bone maturity was improved in the ultrasound group compared to the control group. These results indicate that LIPUS promotes osteoblast differentiation as well as enhances bone ingrowth in porous Ti6AI4V scaffolds, and promotes bone formation and maturity in porous Ti6A14V scaffolds. (C) 2017 Elsevier B.V. All rights reserved.; Low-intensity pulsed ultrasound (LIPUS) has been used in patients with fresh fractures, delayed union and nonunion to enhance bone healing and improve functional outcome. However, there were few studies concerning the effects of LIPUS on the biological behavior of osteoblasts on porous scaffolds. This study aimed to evaluate the effects of LIPUS on the biological behavior of osteoblasts on porous titanium-6aluminum-4vanadium (Ti6A14V) alloy scaffolds in vitro and in vivo. Scaffolds were randomly divided into an ultrasound group and a control group. Mouse pre-osteoblast cells were cultured with porous Ti6AI4V scaffolds in vitro. The effects of LIPUS on the biological behavior of osteoblasts were evaluated by observing the adhesion, proliferation, differentiation and ingrowth depth on porous Ti6A14V scaffolds. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated via micro-CT, fluorescent staining and toluidine blue staining. The results revealed that osteoblast adhered well to the scaffolds, and there was no significant difference in the methyl thiazolyl tetrazolium value between the ultrasound group and the control group (p > 0.05). Compared with the control group, ultrasound promoted the alkaline phosphatase activity, osteocalcin levels and ingrowth depth of the cells on the scaffolds (p < 0.05). In addition, micro-CT and histomorphological analysis showed that the volume and amount of new bone formation were increased and that bone maturity was improved in the ultrasound group compared to the control group. These results indicate that LIPUS promotes osteoblast differentiation as well as enhances bone ingrowth in porous Ti6AI4V scaffolds, and promotes bone formation and maturity in porous Ti6A14V scaffolds. (C) 2017 Elsevier B.V. All rights reserved.
学科主题	Materials Science, bioMaterials
语种	英语
资助机构	National High Technology Research & Development Program (863 Program) of China [2015AA033702]; Natural Science Foundation of Liaoning Province, China [2014021008]
公开日期	2018-01-10
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/79023]
专题	金属研究所_中国科学院金属研究所
通讯作者	Wu, L (reprint author), China Med Univ, Sch Stomatol, Dept Prosthodont, Shenyang, Liaoning, Peoples R China.
推荐引用方式 GB/T 7714	Cao, Hongjuan,Feng, Lifang,Wu, Zhenxian,et al. Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study[J]. ELSEVIER SCIENCE BV,2017,80:7-17.
APA	Cao, Hongjuan.,Feng, Lifang.,Wu, Zhenxian.,Hou, Wentao.,Li, Shujun.,...&Wu, L .(2017).Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study.ELSEVIER SCIENCE BV,80,7-17.
MLA	Cao, Hongjuan,et al."Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study".ELSEVIER SCIENCE BV 80(2017):7-17.