Enhancement of wanlongmycin production by nitrogen ion beam implantation

	Enhancement of wanlongmycin production by nitrogen ion beam implantation
	Zhang, Xiaoyong ; Lin, Birun ; Gao, Xiangyang ; Shen, Huifang
刊名	ELECTRONIC JOURNAL OF BIOTECHNOLOGY
	2010
卷号	13 期号:1 页码:1-6
关键词	mutagen nitrogen ion beam implantation Streptomyces griseovariabilis GAAS2057 wanlongmycin
ISSN号	0717-3458
通讯作者	Lin, BR, Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou 510640, Guangdong, Peoples R China
中文摘要	In an attempt to obtain an industrial strain with higher yield of wanlongmycin, the wild strain Streptomyces griseovariabilis GAAS2507 was mutated by a novel mutagen, nitrogen ion beam with energy of 20 kilo electron volts (KeV) and dose ranging from 7.80 x 10(14) to 2.86 x 10(15) ions/cm(2). One mutant strain WN939 was obtained. Its yield of wanlongmycin reached 271.24 mu g/mL, which was 82.10% higher than that of the wild strain. The mutant strain WN939 was relatively stable for the production of wanlongmycin through six successive transfers of cultures and a repeat fermentation in a 30 L fermentor. In addition, the mutant strains were investigated and divided into five types by their colony phenotypes and production of wanlongmycin. Among them, three types mutant strains exhibited positive mutation, while the other two types mutant strains exhibited negative mutation.
学科主题	Biotechnology & Applied Microbiology
收录类别	SCI
资助信息	National Natural Science Foundation of China [30471161]; Guangdong Natural Science Foundation of China [8151064001000010, 06025388]
语种	英语
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/22510]
专题	成都生物研究所_生态研究
推荐引用方式 GB/T 7714	Zhang, Xiaoyong,Lin, Birun,Gao, Xiangyang,et al. Enhancement of wanlongmycin production by nitrogen ion beam implantation[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2010,13(1):1-6.
APA	Zhang, Xiaoyong,Lin, Birun,Gao, Xiangyang,&Shen, Huifang.(2010).Enhancement of wanlongmycin production by nitrogen ion beam implantation.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,13(1),1-6.
MLA	Zhang, Xiaoyong,et al."Enhancement of wanlongmycin production by nitrogen ion beam implantation".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 13.1(2010):1-6.