Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution

doi:10.1007/s00604-018-2937-9

	Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution
	Tao, LX; Yue, QL; Chen CY(陈春英); Li, CZ; Chen, CY; Wang, YP; Hou, YN
刊名	MICROCHIMICA ACTA
	2018
卷号	185 期号:9 页码:419
关键词	8-OHdG Biomarker Oxidation of DNA Aptamer Conformational change G-quadruplex Magnetic biosensor Reusability Urine samples Air quality index
ISSN号	0026-3672
DOI	10.1007/s00604-018-2937-9
文献子类	Article
英文摘要	An aptamer based method is described for the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG) using resonance light scattering (RLS). Magnetic nanoparticles (MNPs) were employed as RLS probes. The probe DNA was placed on the surface of MNPs, which produces a rather low RLS signal. If, however, probe DNA hybridizes with the aptamer against 8-OHdG, a sandwich structure will be formed. This results in a significant enhancement of RLS intensity. The aptamer was used as the recognition element to capture 8-OHdG. 8-OHdG has a stronger affinity for the aptamer than probe DNA, and the conformation of the aptamer therefore switches from a double-stranded to a G-quadruplex structure. As a result, MNPs labeled with probe DNA are released, and RLS intensity decreases. The method allows 8-OHdG to be detected with a linear response in the 32 pM - 12.0 nM concentration range and an 11 pM limit of detection (at 3.29S (B)/m, according to the recent recommendation of IUPAC). The MNPs can be reused 5 times by applying an external magnetic field for collection. The method was successfully applied to analyze human urine samples for its content of 8-OHdG. It was also found that the levels of 8-OHdG noticeably increased with the increase of the Air Quality Index. Conceivably, the method is a viable tool to investigate the relationship between 8-OHdG levels and the effect of air pollution.
电子版国际标准刊号	1436-5073
WOS关键词	GOLD NANOPARTICLES ; DNA-DAMAGE ; 8-HYDROXY-2-DEOXYGUANOSINE ; NANOCLUSTERS ; SPECTROSCOPY ; AGGREGATION ; BIOSENSOR ; APTAMER ; WORKERS ; CANCER
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000441932100001
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/287034]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Tao, LX,Yue, QL,Chen CY,et al. Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution[J]. MICROCHIMICA ACTA,2018,185(9):419.
APA	Tao, LX.,Yue, QL.,陈春英.,Li, CZ.,Chen, CY.,...&Hou, YN.(2018).Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution.MICROCHIMICA ACTA,185(9),419.
MLA	Tao, LX,et al."Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution".MICROCHIMICA ACTA 185.9(2018):419.