Evidence for molecular antagonistic mechanism between mercury and selenium in rice (Oryza sativa L.): A combined study using 1, 2-dimensional electrophoresis and SR-XRF techniques

doi:10.1016/j.jtemb.2017.10.006

	Evidence for molecular antagonistic mechanism between mercury and selenium in rice (Oryza sativa L.): A combined study using 1, 2-dimensional electrophoresis and SR-XRF techniques
	Li B(李柏); Zhao JT(赵甲亭); Zhang ZY(张智勇); Gao YX(高愈希); Li H(李红); Li YY(李云云); Li YF(李玉锋)
刊名	JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
	2018
卷号	50 页码:435-440
关键词	Mercury Selenium Metalloproteomics Phytotoxicity Molecular mechanism
ISSN号	0946-672X
DOI	10.1016/j.jtemb.2017.10.006
文献子类	Article; Proceedings Paper
英文摘要	Mercury (Hg) is a hazardous chemical in the environment and can accumulate in the food chain. Selenium (Se) is a necessary element for human health and has antagonistic effects on Fig toxicity. In this work, we investigated the effect of Se on Fig containing and Hg-responsive proteins in rice using 1, 2-dimensional electrophoresis combined with SR-XRF techniques. Two weeks old rice seedlings were exposed to Hg and/or Se compounds. After 21 days proteins in the rice roots were separated by electrophoresis and their metal contents were determined by X-ray fluorescence to identify Hg and Se responsive biomolecules. The results show that under Hg stress alone Hg is bound to proteins with molecular weights of 15-25 kDa. With the addition of Se, a new Hg containing protein band in the 55-70 kDa range was also found, while the content of Hg in the 15-25 kDa proteins decreased. Ten and nine new protein spots were identified after adding Se to inorganic Hg and methylmercury exposed roots, respectively. Adding Se regulates the abundance of proteins associated with carbohydrate and energy metabolism, stress response, cell cycle, and DNA replication indicating that these proteins mediate the antagonism of Se against Hg toxicity. This study helps us to better understand the molecular mechanism of Hg tolerance as well as the molecular antagonism between Hg and Se in rice plants.
会议地点	St Petersburg, RUSSIA
WOS关键词	IRON PLAQUE ; METHYLMERCURY PRODUCTION ; WHEAT SEEDLINGS ; ROOT ANATOMY ; PROTEINS ; BIOAVAILABILITY ; RESPONSES ; EXPOSURE ; IDENTIFICATION ; TRANSLOCATION
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
语种	英语
WOS记录号	WOS:000448633900061
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286688]
专题	高能物理研究所_多学科研究中心高能物理研究所_实验物理中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Li B,Zhao JT,Zhang ZY,et al. Evidence for molecular antagonistic mechanism between mercury and selenium in rice (Oryza sativa L.): A combined study using 1, 2-dimensional electrophoresis and SR-XRF techniques[J]. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY,2018,50:435-440.
APA	李柏.,赵甲亭.,张智勇.,高愈希.,李红.,...&李玉锋.(2018).Evidence for molecular antagonistic mechanism between mercury and selenium in rice (Oryza sativa L.): A combined study using 1, 2-dimensional electrophoresis and SR-XRF techniques.JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY,50,435-440.
MLA	李柏,et al."Evidence for molecular antagonistic mechanism between mercury and selenium in rice (Oryza sativa L.): A combined study using 1, 2-dimensional electrophoresis and SR-XRF techniques".JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY 50(2018):435-440.