Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption

doi:10.3390/ijms19030699

	Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption
	Chen K(陈奎); Xing, GM; Wang, ZB; Zhang, JX; Xia, SB; Gu, WH; Chen, K; Qin, YX; Li, J; Chang, YN
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2018
卷号	19 期号:3 页码:699
关键词	fullerenol oocyte meiosis resumption gap junction granulosa cells EGFR/ERK1/2
ISSN号	1422-0067
DOI	10.3390/ijms19030699
文献子类	Article
英文摘要	The excellent biocompatibility and biological effects of fullerenol and its derivatives make their biomedical application promising. The potential effects of fullerenol in mammals have been extensively studied, but little is known about its effects on female reproduction. Using canonical oocyte-granulosa cell complexes (OGCs) in vitro maturation culture model, we investigated the effect of fullerenol on the first oocyte meiotic resumption. In the surrounding granulosa cells, fullerenol nanoparticles occluded the extracellular domain of the epidermal growth factor receptor (EGFR) to reduce EGFR-ligand binding and subsequent extracellular signal-regulated kinase 1 and 2 (ERK1/2) activation, which involved the regulation of connexin 43 (CX43) expression and internalization. Downregulation of CX43 expression and the retraction of transzonal projections (TZPs) interrupted the gap junction channel and TZPs based mass transportation. This effect decreased cyclic adenosine monophosphate (cAMP) levels in the oocyte and thereby accelerated rat oocyte meiosis resumption. Moreover, perinuclear distribution of CX43 and EGFR was observed in granulosa cells, which could further exacerbate the effects. Fullerenol nanoparticles interfered with the strict process of oocyte meiosis resumption, which likely reduced the oocyte quality.
WOS关键词	TITANIUM-DIOXIDE NANOPARTICLES ; SILVER ALLOY NANOPARTICLES ; GAP-JUNCTION ENDOCYTOSIS ; REPRODUCTIVE TOXICITY ; MAMMALIAN OOCYTE ; DEVELOPMENTAL COMPETENCE ; MEIOTIC MATURATION ; GRANULOSA-CELLS ; GRAPHENE OXIDE ; GROWTH-FACTORS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
WOS记录号	WOS:000428309800056
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285992]
专题	高能物理研究所_多学科研究中心高能物理研究所_加速器中心
通讯作者	Xing GM(邢更妹)
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Chen K,Xing, GM,Wang, ZB,et al. Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(3):699.
APA	陈奎.,Xing, GM.,Wang, ZB.,Zhang, JX.,Xia, SB.,...&雷润宏.(2018).Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(3),699.
MLA	陈奎,et al."Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.3(2018):699.