Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing

doi:10.1002/anie.201711690

	Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing
	Wu, HC; Liu, L; Liu, QS; Zhou, K; Sheng, YY; 刘蕾(多); Liu QS(刘全胜); Zhou K(周柯); Guo, BY; Guo BY(郭秉元)
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2018
卷号	57 期号:14 页码:3602-3606
关键词	antibodies biomarkers molecular beacon nanopore sensors quantitative determination
ISSN号	1433-7851
DOI	10.1002/anie.201711690
文献子类	Article
英文摘要	A new nanopore sensing strategy based on triplex molecular beacon was developed for the detection of specific DNA or multivalent proteins. The sensor is composed of a triplex-forming molecular beacon and a stem-forming DNA component that is modified with a host-guest complex. Upon target DNA hybridizing with the molecular beacon loop or multivalent proteins binding to the recognition elements on the stem, the DNA probe is released and produces highly characteristic current signals when translocated through -hemolysin. The frequency of current signatures can be used to quantify the concentrations of the target molecules. This sensing approach provides a simple, quick, and modular tool for the detection of specific macromolecules with high sensitivity and excellent selectivity. It may find useful applications in point-of-care diagnostics with a portable nanopore kit in the future.
电子版国际标准刊号	1521-3773
WOS关键词	ALPHA-HEMOLYSIN NANOPORE ; CANCER-PATIENTS ; NUCLEIC-ACIDS ; SOLID-STATE ; SINGLE ; PROTEIN ; BIOMARKERS ; ANTIBODY ; DISCRIMINATION ; NANOSTRUCTURES
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000428350100008
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285778]
专题	高能物理研究所_多学科研究中心高能物理研究所_核技术应用研究中心
通讯作者	刘蕾(多)
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Wu, HC,Liu, L,Liu, QS,et al. Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018,57(14):3602-3606.
APA	Wu, HC.,Liu, L.,Liu, QS.,Zhou, K.,Sheng, YY.,...&郭秉元.(2018).Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,57(14),3602-3606.
MLA	Wu, HC,et al."Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 57.14(2018):3602-3606.