Complete genome sequence analysis of the fish pathogen Flavobacterium columnare provides insights into antibiotic resistance and pathogenicity related genes

doi:10.1016/j.micpath.2017.08.035

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Complete genome sequence analysis of the fish pathogen Flavobacterium columnare provides insights into antibiotic resistance and pathogenicity related genes
	Zhang, Yulei 1; Zhao, Lijuan 1,2; Chen, Wenjie 2; Huang, Yunmao 2; Yang, Ling 2; Sarathbabu, V.2; Wu, Zaohe 2; Li, Jun 2,4,5; Nie, Pin 3; Lin, Li 1,2,5,6
刊名	MICROBIAL PATHOGENESIS
	2017-10-01
卷号	111 期号:1 页码:203-211
关键词	Flavobacterium columnare Complete genome Antibiotic resistance genes Pathogenicity related genes
ISSN号	0882-4010
DOI	10.1016/j.micpath.2017.08.035
英文摘要	We analyzed here the complete genome sequences of a highly virulent Flavobacterium columnare Pf1 strain isolated in our laboratory. The complete genome consists of a 3,171,081 bp circular DNA with 2784 predicted protein-coding genes. Among these, 286 genes were predicted as antibiotic resistance genes, including 32 RND-type efflux pump related genes which were associated with the export of amino glycosides, indicating inducible aminoglycosides resistances in F. columnare. On the other hand, 328 genes were predicted as pathogenicity related genes which could be classified as virulence factors, gliding motility proteins, adhesins, and many putative secreted proteases. These genes were probably involved in the colonization, invasion and destruction of fish tissues during the infection of E columnare. Apparently, our obtained complete genome sequences provide the basis for the explanation of the interactions between the F. columnare and the infected fish. The predicted antibiotic resistance and pathogenicity related genes will shed a new light on the development of more efficient preventional strategies against the infection of E columnare, which is a major worldwide fish pathogen. (C) 2017 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Fund[31572657] ; National Natural Science Fund[31372563] ; National Natural Science Fund[31602190] ; Administration of Ocean and Fisheries of Guangdong Province[A201512C003] ; Administration of Ocean and Fisheries of Guangdong Province[2015-115] ; Special fund for Science and technology from Hubei Province[2015BBA228] ; Wuhan Science and Technology Bureau[2016020101010089] ; Department of Education of Guangdong Province[KA170500G] ; Chinese Academy of Sciences (CAS)[XDA08010207]
WOS关键词	DEPENDENT CLP PROTEASE ; AC LYASE ACTIVITY ; ESCHERICHIA-COLI ; ACINETOBACTER-BAUMANNII ; LISTERIA-MONOCYTOGENES ; FLEXIBACTER-COLUMNARIS ; KYOTO ENCYCLOPEDIA ; CHANNEL CATFISH ; PURIFICATION ; MEMBRANE
WOS研究方向	Immunology ; Microbiology
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000412960400029
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内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/32661]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者	Zhao, Lijuan; Nie, Pin; Lin, Li
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China 2.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Bree, Guangdong Prov Key Lab Waterfowl Hlth Breeding, Guangzhou 510225, Guangdong, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 4.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI 49783 USA 5.Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 6.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yulei,Zhao, Lijuan,Chen, Wenjie,et al. Complete genome sequence analysis of the fish pathogen Flavobacterium columnare provides insights into antibiotic resistance and pathogenicity related genes[J]. MICROBIAL PATHOGENESIS,2017,111(1):203-211.
APA	Zhang, Yulei.,Zhao, Lijuan.,Chen, Wenjie.,Huang, Yunmao.,Yang, Ling.,...&Lin, Li.(2017).Complete genome sequence analysis of the fish pathogen Flavobacterium columnare provides insights into antibiotic resistance and pathogenicity related genes.MICROBIAL PATHOGENESIS,111(1),203-211.
MLA	Zhang, Yulei,et al."Complete genome sequence analysis of the fish pathogen Flavobacterium columnare provides insights into antibiotic resistance and pathogenicity related genes".MICROBIAL PATHOGENESIS 111.1(2017):203-211.