Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing

doi:10.1186/s12864-017-3824-1

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing
	He, Libo 1; Zhang, Aidi 1; Pei, Yongyan 1,2; Chu, Pengfei 1,2; Li, Yongming 1; Huang, Rong 1; Liao, Lanjie 1; Zhu, Zuoyan 1; Wang, Yaping 1
刊名	BMC GENOMICS
	2017-06-08
卷号	18 页码:15
关键词	Grass carp Grass carp reovirus Transcriptome sequencing Immune response Hemorrhage
ISSN号	1471-2164
DOI	10.1186/s12864-017-3824-1
通讯作者	Wang, Yaping(wangyp@ihb.ac.cn)
英文摘要	Background: Grass carp is an important farmed fish in China that is affected by serious disease, especially hemorrhagic disease caused by grass carp reovirus (GCRV). The mechanism underlying the hemorrhagic symptoms in infected fish remains to be elucidated. Although GCRV can be divided into three distinct subtypes, differences in the pathogenesis and host immune responses to the different subtypes are still unclear. The aim of this study was to provide a comprehensive insight into the grass carp response to different GCRV subtypes and to elucidate the mechanism underlying the hemorrhagic symptoms. Results: Following infection of grass carp, GCRV-I was associated with a long latent period and low mortality (42.5%), while GCRV-II was associated with a short latent period and high mortality (81.4%). The relative copy number of GCRV-I remained consistent or decreased slightly throughout the first 7 days post-infection, whereas a marked increase in GCRV-II high copy number was detected at 5 days post-infection. Transcriptome sequencing revealed 211 differentially expressed genes (DEGs) in Group I (66 up-regulated, 145 down-regulated) and 670 (386 up-regulated, 284 down-regulated) in Group II. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed significant enrichment in the terms or pathways involved in immune responses and correlating with blood or platelets. Most of the DEGs in Group I were also present in Group II, although the expression profiles differed, with most DEGs showing mild changes in Group I, while marked changes were observed in Group II, especially the interferon-related genes. Many of the genes involved in the complement pathway and coagulation cascades were significantly up-regulated at 7 days post-infection in Group II, suggesting activation of these pathways. Conclusion: GCRV-I is associated with low virulence and a long latent period prior to the induction of a mild host immune response, whereas GCRV-II is associated with high virulence, a short latent period and stimulates a strong and extensive host immune response. The complement and coagulation pathways are significantly activated at 7 days post-infection, leading to the endothelial cell and blood cell damage that result in hemorrhagic symptoms.
资助项目	National Natural Science Foundation of China[31130055] ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology[2014FBZ04] ; Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-EW-N-004-3] ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences[Y65E021301]
WOS关键词	HEMATOPOIETIC NECROSIS VIRUS ; COMPLETE GENOME SEQUENCE ; INNATE IMMUNE-RESPONSE ; PROKARYOTIC EXPRESSION ; COMPLEMENT ACTIVATION ; RNA-SEQ ; FISH ; GENE ; INFECTION ; PATHWAY
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000404081100003
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Independent project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/32360]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者	Wang, Yaping
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	He, Libo,Zhang, Aidi,Pei, Yongyan,et al. Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing[J]. BMC GENOMICS,2017,18:15.
APA	He, Libo.,Zhang, Aidi.,Pei, Yongyan.,Chu, Pengfei.,Li, Yongming.,...&Wang, Yaping.(2017).Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing.BMC GENOMICS,18,15.
MLA	He, Libo,et al."Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing".BMC GENOMICS 18(2017):15.