Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry

	Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
	Zhou, Yan ; Lee, Stephanie ; Choi, Franky Fung Kei ; Xu, Gang ; Liu, Xin ; Song, Jing-Zheng ; Li, Song-Lin ; Qiao, Chun-Feng ; Xu, Hong-Xi
刊名	ANALYTICA CHIMICA ACTA
	2010
卷号	678 期号:1 页码:96-107
关键词	Polycyclic polyprenylated acylphloroglucinols (PPAPs) Garcinia species Ultra performance liquid chromatography Quadrupole time-of-flight mass spectrometry
ISSN号	0003-2670
通讯作者	Xu, HX, Hong Kong Jockey Club Inst Chinese Med, Bioinformat Ctr, Unit 703, 7th Flr,2 Sci Pk,Hong Kong Sci Pk, Shatin, Hong Kong, Peoples R China
产权排序	2
中文摘要	Polycyclic polyprenylated acylphloroglucinols (PPAPs) are a group of natural products isolated from different Garcinia species with a wide range of important biological activities. In this study, an ultra performance liquid chromatography (UPLC) coupled to photodiode-array detection and quadrupole time-of-flight mass spectrometry (Q-TOF) method was developed to characterize 16 PPAPs in 10 Garcinia species. In source dissociation techniques based on cone voltage fragmentation were used to fragment the deprotonated molecules and multiple mass spectrometry (MS/MS) using ramping collision energy were used to further break down the resulting product ions. The resulting characteristic fragment ions were generated by cleavage of C1-C5 bond and C7-C8 bond through concerted pericyclic reaction, which is especially valuable for differentiating three types of PPAPs isomers. As such, two new PPAPs isomers present in minor amount in the extracts of Garcinia oblongifolia were tentatively characterized by comparing their tandem mass spectra to the known ones. In addition, an UPLC-Q-TOF-MS method was validated for the quantitative determination of PPAPs. The method exhibited limits of detection from 2.7 to 21.4 ng mL(-1) and intra-day and inter-day variations were less than 3.7% and the recovery was in the range of 89-107% with RSD less than 9.0%. This UPLC-Q-TOF-MS method has successfully been applied to quantify 16 PPAPs in 32 samples of 10 Garcinia species, which were found to be a rich source of PPAPs. (C) 2010 Elsevier B.V. All rights reserved.
学科主题	Chemistry, Analytical
收录类别	SCI
资助信息	Hong Kong Jockey Club Charities Trust Fund
语种	英语
WOS记录号	WOS:000282850800014
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/18423]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Zhou, Yan,Lee, Stephanie,Choi, Franky Fung Kei,et al. Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2010,678(1):96-107.
APA	Zhou, Yan.,Lee, Stephanie.,Choi, Franky Fung Kei.,Xu, Gang.,Liu, Xin.,...&Xu, Hong-Xi.(2010).Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.ANALYTICA CHIMICA ACTA,678(1),96-107.
MLA	Zhou, Yan,et al."Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry".ANALYTICA CHIMICA ACTA 678.1(2010):96-107.