Analysis of Saussurea Species from Tibet Using HPLC-DAD-ESI-MSn

	Analysis of Saussurea Species from Tibet Using HPLC-DAD-ESI-MSn
	Dawa, Z. ; Zhou, Y. ; Bai, Y. ; Gesang, S. ; Liang, J. ; Ding, L.
刊名	ACTA CHROMATOGRAPHICA
	2010
卷号	22 期号:1 页码:141-152
关键词	HPLC-DAD-ESI-MSn analysis flavonoid coumarin Saussurea species Tibet
ISSN号	1233-2356
通讯作者	Zhou, Y, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
产权排序	2
中文摘要	An HPLC-DAD-ESI-MSn method has been developed for simultaneous quantification of eight major compounds in eight Saussurea species which have long been used as the traditional Tibetan medicines. The method was validated for sensitivity, precision, and accuracy. LODs were from 0.11 to 5.01 mu g mL(-1), overall intra-day and inter-day variation was less than 2.70%, and overall recovery was over 98.0%. The correlation coefficients (r(2)) of the calibration plots were >0.991. This newly established method was successfully used to reveal difference among the chemical profiles and analytes contents of eight Saussurea species collected in Tibet. In addition, by comparison of UV and mass spectra with those of authentic compounds, a total of fifteen peaks were identified. It can be concluded that this is an effective method for quantification and evaluation of the flavonoids and coumarins in the eight species of the genus Saussurea. It can be used as an efficient reference method for development and use of the eight traditional Tibetan medicines by comparing their different characteristics.
学科主题	Chemistry, Analytical
收录类别	SCI
资助信息	National Key Technology R&D Program of China [2007BAI31B02]
语种	英语
WOS记录号	WOS:000279636600011
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/18369]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Dawa, Z.,Zhou, Y.,Bai, Y.,et al. Analysis of Saussurea Species from Tibet Using HPLC-DAD-ESI-MSn[J]. ACTA CHROMATOGRAPHICA,2010,22(1):141-152.
APA	Dawa, Z.,Zhou, Y.,Bai, Y.,Gesang, S.,Liang, J.,&Ding, L..(2010).Analysis of Saussurea Species from Tibet Using HPLC-DAD-ESI-MSn.ACTA CHROMATOGRAPHICA,22(1),141-152.
MLA	Dawa, Z.,et al."Analysis of Saussurea Species from Tibet Using HPLC-DAD-ESI-MSn".ACTA CHROMATOGRAPHICA 22.1(2010):141-152.