Orange protein has a role in phytoene synthase stabilization in sweetpotato

doi:10.1038/srep33563

	Orange protein has a role in phytoene synthase stabilization in sweetpotato
	Park, Seyeon 1,2; Kim, Ho Soo 1; Jung, Young Jun 3,4; Kim, Sun Ha 1; Ji, Chang Yoon 1,2; Wang, Zhi 5; Jeong, Jae Cheol 1,2; Lee, Haeng-Soon 1,2; Lee, Sang Yeol 3; Kwak, Sang-Soo 1,2
刊名	SCIENTIFIC REPORTS
	2016-09-16
卷号	6 页码:12
ISSN号	2045-2322
DOI	10.1038/srep33563
通讯作者	Kwak, Sang-Soo(sskwak@kribb.re.kr)
英文摘要	Carotenoids have essential roles in light-harvesting processes and protecting the photosynthetic machinery from photo-oxidative damage. Phytoene synthase (PSY) and Orange (Or) are key plant proteins for carotenoid biosynthesis and accumulation. We previously isolated the sweetpotato (Ipomoea batatas) Or gene (IbOr), which is involved in carotenoid accumulation and salt stress tolerance. The molecular mechanism underlying IbOr regulation of carotenoid accumulation was unknown. Here, we show that IbOr has an essential role in regulating IbPSY stability via its holdase chaperone activity both in vitro and in vivo. This protection results in carotenoid accumulation and abiotic stress tolerance. IbOr transcript levels increase in sweetpotato stem, root, and calli after exposure to heat stress. IbOr is localized in the nucleus and chloroplasts, but interacts with IbPSY only in chloroplasts. After exposure to heat stress, IbOr predominantly localizes in chloroplasts. IbOr overexpression in transgenic sweetpotato and Arabidopsis conferred enhanced tolerance to heat and oxidative stress. These results indicate that IbOr holdase chaperone activity protects IbPSY stability, which leads to carotenoid accumulation, and confers enhanced heat and oxidative stress tolerance in plants. This study provides evidence that IbOr functions as a molecular chaperone, and suggests a novel mechanism regulating carotenoid accumulation and stress tolerance in plants.
资助项目	National Research Foundation of Korea (NRF) - Korean Government[2015053321] ; Systems & Synthetic Agrobiotech Center[PJ01106401] ; Biogreen 21 Project for Next Generation, Rural Development Administration, Korea ; KRIBB initiative program
WOS关键词	SALT-STRESS TOLERANCE ; ZINC-FINGER DOMAIN ; ARABIDOPSIS-THALIANA ; BETA-CAROTENE ; HEAT-SHOCK ; MOLECULAR CHAPERONES ; DOWN-REGULATION ; ABIOTIC STRESS ; PROTOCHLOROPHYLLIDE OXIDOREDUCTASE ; ENHANCES TOLERANCE
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000383649900001
资助机构	National Research Foundation of Korea (NRF) - Korean Government ; Systems & Synthetic Agrobiotech Center ; Biogreen 21 Project for Next Generation, Rural Development Administration, Korea ; KRIBB initiative program
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/65900]
专题	中国科学院地理科学与资源研究所
通讯作者	Kwak, Sang-Soo
作者单位	1.KRIBB, Plant Syst Engn Res Ctr, 125 Gwahak Ro, Daejeon 34141, South Korea 2.Korea Univ Sci & Technol UST, Dept Green Chem & Environm Biotechnol, 217 Gajeong Ro, Daejeon 34113, South Korea 3.Gyeongsang Natl Univ, Div Appl Life Sci, Plus Program BK21, 501 Jinjudae Ro, Jinju 52828, South Korea 4.Natl Inst Ecol, 1210 Geumgang Ro, Maseo Myeon 33657, Seocheon Gun, South Korea 5.Northwest A&F Univ, Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Xianyang 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Park, Seyeon,Kim, Ho Soo,Jung, Young Jun,et al. Orange protein has a role in phytoene synthase stabilization in sweetpotato[J]. SCIENTIFIC REPORTS,2016,6:12.
APA	Park, Seyeon.,Kim, Ho Soo.,Jung, Young Jun.,Kim, Sun Ha.,Ji, Chang Yoon.,...&Kwak, Sang-Soo.(2016).Orange protein has a role in phytoene synthase stabilization in sweetpotato.SCIENTIFIC REPORTS,6,12.
MLA	Park, Seyeon,et al."Orange protein has a role in phytoene synthase stabilization in sweetpotato".SCIENTIFIC REPORTS 6(2016):12.