Study on the reaction of proteinase from bacillus subtilis with Alizarin Complex | |
Wang, XM ; Dong, FQ ; Ding, LS ; Yang, DM ; Shi, RM | |
刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS |
2005 | |
卷号 | 25期号:9页码:1471_1474 |
关键词 | bacillus subtilis proteinase Alizarin complex effect mechanism UV spectrometry dialysis method |
ISSN号 | 1000-0593 |
通讯作者 | Wang, XM, SW Univ Sci & Technol, Dept Biol & Chem Engn, Mianyang 621010, Peoples R China. |
产权排序 | 2 |
中文摘要 | The interaction of bacillus subtilis proteinase(BSP) and Alizarin Complex(ALC) was investigated by spectrophotometric method in acidic solution(pH 4.20). When BSP was added into ALC, a red color was observed, which indicated the formation of BSP-ALC associated compound. The maximum absorption of the red color coordination compound was obtained at 510 mn. It was found that the red shift of maximum absorption of the complex was 85 rim. According to balance percolation method, molar ratio method and double wavelengh method, the apparent molar absorptivity epsilon(B) = 6.68 X 10(3) L center dot mol(-1) center dot cm(-1). Conditional constants were defined, the. maximum binding number n = 10, and the apparent binding constant K = 7.25 x 10(6) L center dot mol(-1). It was found that Scatchard model is appropriate in the treatment of data obtained here. |
学科主题 | Spectroscopy |
收录类别 | SCI |
语种 | 中文 |
WOS记录号 | WOS:000232296200025 |
公开日期 | 2011-07-08 |
内容类型 | 期刊论文 |
源URL | [http://210.75.237.14/handle/351003/17669] |
专题 | 成都生物研究所_天然产物研究 |
推荐引用方式 GB/T 7714 | Wang, XM,Dong, FQ,Ding, LS,et al. Study on the reaction of proteinase from bacillus subtilis with Alizarin Complex[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2005,25(9):1471_1474. |
APA | Wang, XM,Dong, FQ,Ding, LS,Yang, DM,&Shi, RM.(2005).Study on the reaction of proteinase from bacillus subtilis with Alizarin Complex.SPECTROSCOPY AND SPECTRAL ANALYSIS,25(9),1471_1474. |
MLA | Wang, XM,et al."Study on the reaction of proteinase from bacillus subtilis with Alizarin Complex".SPECTROSCOPY AND SPECTRAL ANALYSIS 25.9(2005):1471_1474. |
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