Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties

doi:10.1016/j.ijbiomac.2019.03.246

	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties
	Wang, Yonggang 1,2; Li, Yuanli 1; Li, Shaowei 3; Li, QingYang 4; Fan, Wenguang 2; Kiatoukosin, Lormoua 2; Chen, Jixiang 1,5
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2019-07-15
卷号	133 页码:172-183
关键词	Endophytic fungus exopolysaccharide Production optimization and purification Structure and bioactivities
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2019.03.246
通讯作者	Wang, Yonggang(412316788@163.com) ; Chen, Jixiang(chenjixiang@lut.cn)
英文摘要	The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways. An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima Fl. The production optimization, purification and characterization of the purified EPS from A. tenuissima Fl and the in-vitro antioxidant potentials was investigated. Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4. 7H20, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 C, inoculum size of 3%, 180 r/min for 5 d. The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 C by thermogravimetric analysis (TG/DSC). Molecular weight of the purified EPS was 3.246 x 104 g/mol, and EPS was composed of o-galacturonic acid, rhamnose, o-mannose, glucose, and o-galactose in ratio of 0.45:3.02:3.25:1.0:0.95. The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[31760028] ; National Natural Science Foundation of China[31460032] ; Youth Talent Support Program of Lanzhou University of Technology[2018]
WOS关键词	STRUCTURAL-CHARACTERIZATION ; EXOPOLYSACCHARIDE PRODUCTION ; OPTIMIZATION ; CULTURE ; ANTITUMOR ; EXTRACTION ; PULLULAN
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000472685700021
资助机构	National Natural Science Foundation of China ; Youth Talent Support Program of Lanzhou University of Technology
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/58404]
专题	中国科学院地理科学与资源研究所
通讯作者	Wang, Yonggang; Chen, Jixiang
作者单位	1.Lanzhou Univ Technol, Sch Energy & Power Engn, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 3.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China 4.Cent S Univ, Xiangya Sch Med, Changsha 410000, Hunan, Peoples R China 5.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yonggang,Li, Yuanli,Li, Shaowei,et al. Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,133:172-183.
APA	Wang, Yonggang.,Li, Yuanli.,Li, Shaowei.,Li, QingYang.,Fan, Wenguang.,...&Chen, Jixiang.(2019).Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,133,172-183.
MLA	Wang, Yonggang,et al."Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 133(2019):172-183.