Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 广东省海洋药物重点实验室

	Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture
	sfchen@hkusua.hku.hk; Ip, PF; Chen, F
刊名	PROCESS BIOCHEMISTRY
	2005
卷号	40 期号:11 页码:3491-3496
关键词	Chlorella Zofingiensis Heterotrophic Culture Secondary Carotenoids Astaxanthin Oxidative Stress Reactive Oxygen Species
ISSN号	1359-5113
英文摘要	The advantages of using heterotrophic cultivation for commercial production of the high-value ketocarotenoid astaxanthin by microalgae have been emphasized in recent years [Chen F. High cell density culture of microalgae in heterotrophic growth. Trends Biotechnol 1996;14:421-5; Chen F, Chen H, Gong X. Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions. Bioresour Technol 1997;62:19-24; Ip, PF, Chen F. Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark. Process Biochem 2005;40:733-8]. In a heterotrophic culture where light is not provided, the employment of oxidative stress is essential for promoting the formation of secondary carotenoids including astaxanthin. In the present study, oxidative treatment imposed by chemically generated reactive oxygen species (ROS) including the hydroxyl radicals ((OH)-O-center dot) and singlet oxygen (O-1(2)) for inducing carotenogenesis and astaxanthin production by C. zofingiensis in heterotrophic culture was investigated. (OH)-O-center dot stimulated secondary carotenoids accumulation significantly, whereas O-1(2) did not. The addition of 0.1 mM hydrogen peroxide to the culture leading to the generation of (OH)-O-center dot enhanced the yield of astaxanthin from 9.9 mg l(-1) to 12.58 mg l(-1). These results indicate that the application of a suitable type and dosage of ROS is potentially useful for mass production of astaxanthin by C. zofingiensis in heterotrophic culture. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
资助项目	广东省海洋药物重点实验室
WOS记录号	WOS:000232393700015
公开日期	2011-06-29
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/2221]
专题	南海海洋研究所_广东省海洋药物重点实验室
通讯作者	sfchen@hkusua.hku.hk
推荐引用方式 GB/T 7714	sfchen@hkusua.hku.hk,Ip, PF,Chen, F. Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture[J]. PROCESS BIOCHEMISTRY,2005,40(11):3491-3496.
APA	sfchen@hkusua.hku.hk,Ip, PF,&Chen, F.(2005).Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture.PROCESS BIOCHEMISTRY,40(11),3491-3496.
MLA	sfchen@hkusua.hku.hk,et al."Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture".PROCESS BIOCHEMISTRY 40.11(2005):3491-3496.