IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE

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	IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE
	He, MX; hmx@scsio.ac.cn; Huang, LM; Yuan, T
刊名	JOURNAL OF SHELLFISH RESEARCH
	2012
卷号	31 期号:1 页码:33-37
关键词	Scallop Chlamys Nobilis Reeve Aflp Shell Color Genetic Control
ISSN号	0730-8000
英文摘要	The DNA amplified fragment length polymorphism (AFLP) fingerprinting approach was used to identify a shell color-linked marker in the noble scallop Chlamys nobilis Reeve. The marker BIfI76 was linked completely to the gene for brigh-torange shell color with no recombination in the progeny we sampled. This marker was amplified in bright-orange parents and all orange progeny, but was absent in all of the brownish purple progeny. Nevertheless, A FLP markers are not feasible for large-scale application. Thus, we converted BIfI76 into a sequence-characterized amplified region (SCAR) marker that we designated as SCAR Orange-142. The marker Orange-142, like the A FLP band from which it was derived, amplified a robust band in only the bright-orange scallops, both in F, and F-2 families as well as in a test group obtained from a seafood market. These results indicate that Orange-142 is tightly linked to the gene for orange shell color. In all but 1 of 10 F2 families derived from bright-orange F-o grandparents, the observed frequencies of orange progeny were consistent with Mendelian ratios expected from a I-locus 2-allele system in which the brownish purple genotype was recessive. This study is the first report of the genetic basis for shell color in C. nobilis. The identification of a shell color-linked marker in the noble scallop improves our understanding of shell color inheritance and may contribute to C. nobilis broodstock enhancement programs using marker-assisted selection.
学科主题	Fisheries ; Marine & Freshwater Biology
资助项目	LMB
语种	英语
WOS记录号	WOS:000302846800004
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9948]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	hmx@scsio.ac.cn
推荐引用方式 GB/T 7714	He, MX,hmx@scsio.ac.cn,Huang, LM,et al. IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE[J]. JOURNAL OF SHELLFISH RESEARCH,2012,31(1):33-37.
APA	He, MX,hmx@scsio.ac.cn,Huang, LM,&Yuan, T.(2012).IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE.JOURNAL OF SHELLFISH RESEARCH,31(1),33-37.
MLA	He, MX,et al."IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE".JOURNAL OF SHELLFISH RESEARCH 31.1(2012):33-37.