Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides)

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	Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides)
	qinqw@scsio.ac.cn; Guo, ML; Wei, JG; Huang, XH; Qin, QW; Huang, YH
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012
卷号	32 期号:5 页码:828-838
关键词	Beta-defensin Antiviral Effects Epinephelus Coioides Sgiv Vnnv
ISSN号	1050-4648
英文摘要	Defensins are a group of small antimicrobial peptides playing an important role in innate host defense. In this study, a beta-defensin cloned from liver of orange-spotted grouper, Epinephelus coioides, EcDefensin, showed a key role in inhibiting the infection and replication of two kinds of newly emerging marine fish viruses, an enveloped DNA virus of Singapore grouper iridovirus (SGIV), and a non-enveloped RNA virus of viral nervous necrosis virus (VNNV). The expression profiles of EcDefensin were significantly (P < 0.001) up-regulated after challenging with Lipopolysaccharide (LPS), SGIV and Polyriboinosinic Polyribocytidylic Acid (polyl:C) in vivo. Immunofluorescence staining observed its intracellular innate immune response to viral infection of SGIV and VNNV. EcDefensin was found to possess dual antiviral activity, inhibiting the infection and replication of SGIV and VNNV and inducting a type I interferon-related response in vitro. Synthetic peptide of EcDefensin (Ec-defensin) incubated with virus or cells before infection reduced the viral infectivity. Ec-defensin drastically decreased SGIV and VNNV titers, viral gene expression and structural protein accumulation. Grouper spleen cells over-expressing EcDefensin (GS/pcDNA-EcDefensin) support the inhibition of viral infection and the upregulation of the expression of host immune-related genes, such as antiviral protein Mx and pro-inflammatory cytokine IL-1 beta. EcDefensin activated type I IFN and Interferon-sensitive response element (ISRE) in vitro. Reporter genes of IFN-Luc and ISRE-Luc were significantly up-regulated in cells transfected with pcDNA-EcDefenisn after infection with SGIV and VNNV. These results suggest that EcDefensin is importantly involved in host immune responses to invasion of viral pathogens, and open the new avenues for design of antiviral agents in fisheries industry. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助项目	LMB
语种	英语
WOS记录号	WOS:000302667200025
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9934]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	qinqw@scsio.ac.cn
推荐引用方式 GB/T 7714	qinqw@scsio.ac.cn,Guo, ML,Wei, JG,et al. Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(5):828-838.
APA	qinqw@scsio.ac.cn,Guo, ML,Wei, JG,Huang, XH,Qin, QW,&Huang, YH.(2012).Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,32(5),828-838.
MLA	qinqw@scsio.ac.cn,et al."Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 32.5(2012):828-838.